Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus

Takafumi Tomura; Hiroko Kishino; Katsumi Doi; Toshio Hara; Seiya Ogata; Satoru Kuhara

doi:10.1271/bbb.57.438

Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus

Takafumi Tomura, Hiroko Kishino, Katsumi Doi, Toshio Hara, Seiya Ogata, Satoru Kuhara

Systems Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

A plasmid, pSA1.1, of Streptomyces azureus elicited conjugative pocks and inhibited the sporulation of its host mycelia. pSA1.1 was studied with a kanamycin resistance gene derived from S. kanamyceticus as a selective marker. The deletion analysis and sequencing of the derivative plasmid found an open reading frame (ORF909b) that was involved in the sporulation-inhibitory function of pSA1.1 and in pock formation. The nucleotide sequences of ORF909b were compared with those of the genes registered in GenBank, but no similarity was found. However, the predicted amino acid sequence of ORF909b showed a significantly high similarity with that of the spoIIIE gene of Bacillus subtilis. This detected gene might be a new sporulation-regulatory gene in streptomycetes.

Original language	English
Pages (from-to)	438-443
Number of pages	6
Journal	Bioscience, biotechnology, and biochemistry
Volume	57
Issue number	3
DOIs	https://doi.org/10.1271/bbb.57.438
Publication status	Published - 1993

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus",

abstract = "A plasmid, pSA1.1, of Streptomyces azureus elicited conjugative pocks and inhibited the sporulation of its host mycelia. pSA1.1 was studied with a kanamycin resistance gene derived from S. kanamyceticus as a selective marker. The deletion analysis and sequencing of the derivative plasmid found an open reading frame (ORF909b) that was involved in the sporulation-inhibitory function of pSA1.1 and in pock formation. The nucleotide sequences of ORF909b were compared with those of the genes registered in GenBank, but no similarity was found. However, the predicted amino acid sequence of ORF909b showed a significantly high similarity with that of the spoIIIE gene of Bacillus subtilis. This detected gene might be a new sporulation-regulatory gene in streptomycetes.",

author = "Takafumi Tomura and Hiroko Kishino and Katsumi Doi and Toshio Hara and Seiya Ogata and Satoru Kuhara",

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T1 - Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus

AU - Tomura, Takafumi

AU - Kishino, Hiroko

AU - Doi, Katsumi

AU - Hara, Toshio

AU - Ogata, Seiya

AU - Kuhara, Satoru

PY - 1993

Y1 - 1993

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AB - A plasmid, pSA1.1, of Streptomyces azureus elicited conjugative pocks and inhibited the sporulation of its host mycelia. pSA1.1 was studied with a kanamycin resistance gene derived from S. kanamyceticus as a selective marker. The deletion analysis and sequencing of the derivative plasmid found an open reading frame (ORF909b) that was involved in the sporulation-inhibitory function of pSA1.1 and in pock formation. The nucleotide sequences of ORF909b were compared with those of the genes registered in GenBank, but no similarity was found. However, the predicted amino acid sequence of ORF909b showed a significantly high similarity with that of the spoIIIE gene of Bacillus subtilis. This detected gene might be a new sporulation-regulatory gene in streptomycetes.

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Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces Azureus

Abstract

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