We have developed a new methodology to fabricate high resolution DNA microarray (2,500-10<Sup>4</Sup> dots/cm<Sup>2</Sup>) in combination with micro contact printing (μ-CP) and SPR imaging. Novel COOH-terminated PEG-disulfides with <I>p</I>-carborane were synthesized to fabricate the first layer on gold substrates to achieve surface coupling reaction with amino-terminated DNA in high yield. The hybridization of the target-DNA modified gold nanoparticles on probe-DNA patterned surfaces was successfully demonstrated by the SPR imaging, where nonspecific adsorption was not observed to the array background. The gold nanoparticle arrays provide quite high contrast even at low surface coverage (∼10%) by the enhancement effect of optical signals based on nano-scale phenomena in the near field. This enhancement effect can be well demonstrated by the simulation based on Maxwell-Garnett (MG) theory and Fresnel's equation.
|Translated title of the contribution||Biosensor Device with SPR Imaging Technique: The Design of High Density DNA Bio-tip|
|Number of pages||6|
|Journal||表面科学 : hyomen kagaku = Journal of the Surface Science Society of Japan|
|Publication status||Published - Jan 10 2006|