Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'

Yoshizumi Ishino, Yosiko Nomura, Ikunoshin Kato

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.

Original languageEnglish
Pages (from-to)742-744
Number of pages3
JournalNucleic acids research
Volume23
Issue number5
DOIs
Publication statusPublished - Mar 11 1995
Externally publishedYes

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Type II Site Specific Deoxyribonucleases
Streptomyces
DNA
Adenoviridae
Bacteriophages
Enzymes
AG 3-5

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'. / Ishino, Yoshizumi; Nomura, Yosiko; Kato, Ikunoshin.

In: Nucleic acids research, Vol. 23, No. 5, 11.03.1995, p. 742-744.

Research output: Contribution to journalArticle

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