Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

Shinichi Kuriyama; Yasushi Taguchi; Kazuto Watanabe; Kanako Nishimura; Kazutoshi Yanagibashi; Yoshiki Katayama; Takuro Niidome

doi:10.1016/j.bmc.2009.09.049

Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

Shinichi Kuriyama, Yasushi Taguchi, Kazuto Watanabe, Kanako Nishimura, Kazutoshi Yanagibashi, Yoshiki Katayama, Takuro Niidome

Materials Science and Engineering, School of Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.

Original language	English
Pages (from-to)	7643-7646
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry
Volume	17
Issue number	22
DOIs	https://doi.org/10.1016/j.bmc.2009.09.049
Publication status	Published - Nov 15 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex. / Kuriyama, Shinichi; Taguchi, Yasushi; Watanabe, Kazuto; Nishimura, Kanako; Yanagibashi, Kazutoshi; Katayama, Yoshiki; Niidome, Takuro.

In: Bioorganic and Medicinal Chemistry, Vol. 17, No. 22, 15.11.2009, p. 7643-7646.

Research output: Contribution to journal › Article › peer-review

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AU - Katayama, Yoshiki

AU - Niidome, Takuro

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