Stability of mutant actins

Douglas Robert Drummond, E. S. Hennessey, J. C. Sparrow

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.

Original languageEnglish
Pages (from-to)301-303
Number of pages3
JournalBiochemical Journal
Volume274
Issue number1
DOIs
Publication statusPublished - Jan 1 1991

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Actins
Drosophila
Urea
Electrophoresis
Gels
Genes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Drummond, D. R., Hennessey, E. S., & Sparrow, J. C. (1991). Stability of mutant actins. Biochemical Journal, 274(1), 301-303. https://doi.org/10.1042/bj2740301

Stability of mutant actins. / Drummond, Douglas Robert; Hennessey, E. S.; Sparrow, J. C.

In: Biochemical Journal, Vol. 274, No. 1, 01.01.1991, p. 301-303.

Research output: Contribution to journalArticle

Drummond, DR, Hennessey, ES & Sparrow, JC 1991, 'Stability of mutant actins', Biochemical Journal, vol. 274, no. 1, pp. 301-303. https://doi.org/10.1042/bj2740301
Drummond, Douglas Robert ; Hennessey, E. S. ; Sparrow, J. C. / Stability of mutant actins. In: Biochemical Journal. 1991 ; Vol. 274, No. 1. pp. 301-303.
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