Stable retinal gene expression in nonhuman primates via subretinal injection of SIVagm-based lentiviral vectors

Yasuhiro Ikeda, Yoshikazu Yonemitsu, Masanori Miyazaki, Ri Ichiro Kohno, Yusuke Murakami, Toshinori Murata, Toshiaki Tabata, Yasuji Ueda, Fumiko Ono, Toshimichi Suzuki, Naohide Ageyama, Keiji Terao, Mamoru Hasegawa, Katsuo Sueishi, Tatsuro Ishibashi

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Gene therapy may hold promise as a therapeutic approach for the treatment of intractable ocular diseases, including retinitis pigmentosa (RP). Gene transfer vectors that are able to show long-lasting transgene expression in vivo are highly desirable to treat RP; however, there is a dearth of information regarding long-term transgene expression in the eyes of large animals. We previously reported that the simian immunodeficiency virus from African green monkeys (SIVagm)-based lentiviral vector showed efficient, stable, and safe retinal gene transfer, resulting in significant prevention of retinal degeneration by gene transfer of a neurotrophic factor, human pigment epithelium-derived factor (hPEDF), in rodents. Before applying this strategy in a clinical setting, we here assessed the long-lasting transgene expression of our third-generation SIVagm-based lentiviral vectors in the retinal tissue of nonhuman primates. Approximately 20-50μl of SIV-EGFP (enhanced green fluorescent protein) or SIV-hPEDF was injected into the subretinal space via a glass capillary tube. To detect EGFP expression in the retina, we used a fluorescence fundus camera at various time points after gene transfer. Human PEDF expression was assessed by immunohistochemical analysis, Western blot assay, and enzyme-linked immunosorbent assay. The retinas demonstrated frequent EGFP expression that was preserved for at least 4 years without significant decline. The expression of hPEDF was stable, and occurred mainly in the retinal pigment epithelium. The secreted protein was detected in vitreous and aqueous humor. We thus propose that SIVagm-mediated stable gene transfer might be significantly useful for ocular gene transfer in a clinical setting.

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalHuman Gene Therapy
Volume20
Issue number6
DOIs
Publication statusPublished - Jun 1 2009

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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