Stacking-cyclodextrin-microchip electrokinetic chromatographic determination of gabapentinoid drugs in pharmaceutical and biological matrices

Abdallah M. Zeid, Noritada Kaji, Jenny Jeehan M. Nasr, Fathalla F. Belal, Yoshinobu Baba, Mohamed I. Walash

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A facile, rapid, and highly sensitive microchip-based electrokinetic chromatographic method was developed for the simultaneous analysis of two gabapentinoid drugs, gabapentin (GPN) and pregabalin (PGN). Both drugs were first reacted with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) via nucleophilic substitution reactions to yield highly fluorescent products with λex/em 470/540 nm. Analyses of both fluorescently labeled compounds were achieved within 200 s in a poly(methyl methacrylate) (PMMA) microchip with a 30 mm separation channel. Optimum separation was achieved using a borate buffer (pH 9.0) solution containing methylcellulose and β-cyclodextrin (β-CD) as buffer additives. Methylcellulose acted as a dynamic coating to prevent adsorption of the studied compounds on the inner surfaces of the microchannels, while β-CD acted as a pseudo-stationary phase to improve the separation efficiency between the labeled drugs with high resolution (Rs > 7). The fluorescence intensities of the labeled drugs were measured using a light emitting diode-induced fluorescence detector at 540 nm after excitation at 470 nm. The sensitivity of the method was enhanced 14- and 17-fold for PGN and GPN, respectively by field-amplified stacking relative to traditional pinched injection so that it could quantify 10 ng mL−1 for both analytes, with a detection limit lower than 3 ng mL−1. The developed method was efficiently applied to analyze PGN and GPN in their pharmaceutical dosage forms and in biological fluids. The extraction recoveries of the studied drugs from plasma and urine samples were more than 89% with%RSD values lower than 6.2.

Original languageEnglish
Pages (from-to)65-75
Number of pages11
JournalJournal of Chromatography A
Volume1503
DOIs
Publication statusPublished - Jun 23 2017

Fingerprint

Cyclodextrins
Pharmaceutical Preparations
Methylcellulose
Buffers
Fluorescence
Borates
Dosage Forms
Polymethyl Methacrylate
Microchannels
Adsorption
Light emitting diodes
Limit of Detection
Substitution reactions
Urine
Detectors
Plasmas
Light
Recovery
Coatings
Injections

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Stacking-cyclodextrin-microchip electrokinetic chromatographic determination of gabapentinoid drugs in pharmaceutical and biological matrices. / Zeid, Abdallah M.; Kaji, Noritada; Nasr, Jenny Jeehan M.; Belal, Fathalla F.; Baba, Yoshinobu; Walash, Mohamed I.

In: Journal of Chromatography A, Vol. 1503, 23.06.2017, p. 65-75.

Research output: Contribution to journalArticle

Zeid, Abdallah M. ; Kaji, Noritada ; Nasr, Jenny Jeehan M. ; Belal, Fathalla F. ; Baba, Yoshinobu ; Walash, Mohamed I. / Stacking-cyclodextrin-microchip electrokinetic chromatographic determination of gabapentinoid drugs in pharmaceutical and biological matrices. In: Journal of Chromatography A. 2017 ; Vol. 1503. pp. 65-75.
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