Staining ability and biocompatibility of brilliant blue G

Preclinical study of brilliant blue G as an adjunct for capsular staining

Toshio Hisatomi, Hiroshi Enaida, Hiroyoshi Matsumoto, Tadahisa Kagimoto, Akifumi Ueno, Yasuaki Hata, Toshiaki Kubota, Yoshinobu Goto, Tatsuro Ishibashi

Research output: Contribution to journalArticle

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Abstract

Objective: To evaluate the effectiveness and biocompatibility of brilliant blue G (BBG) for capsular visualization for continuous curvilinear capsulorrhexis. Methods: The capsular staining ability of BBG was evaluated at graded concentrations of 10.0, 1.0, 0.5, 0.25, 0.1, and 0.01 mg/mL in enucleated pig's eyes. The biocompatibility of BBG was assessed in rat's eyes for 2 months. The eyes were analyzed using light, fluorescence, transmission electron, and scanning electron microscopy. TUNEL (terminal deoxynucleotidyl transferase-mediated biotin-deoxyuridine triphosphate nick-end labeling) was used to detect apoptotic cells, and endothelial cell counts were analyzed using scanning electron microscopy. The results were compared using indocyanine green and trypan blue. Results: The BBG improved capsular visualization, and a complete capsulorrhexis could be performed. In the rat model, no apparent toxic effect was observed using biomicroscopy during 2 months. Histologically, BBG showed satisfactory biocompatibility. Apoptotic cell death of the endothelial cells was detected in only the trypan blue group. In contrast to BBG, indocyanine green and trypan blue showed degeneration of corneal endothelial cells using transmission and scanning electron microscopy. Conclusion: The BBG contributed to better capsular visualization and caused no apparent complications to the corneal endothelium. Clinical Relevance: The BBG is effective and safe capsular staining for continuous curvilinear capsulorrhexis.

Original languageEnglish
Pages (from-to)514-519
Number of pages6
JournalArchives of Ophthalmology
Volume124
Issue number4
DOIs
Publication statusPublished - Apr 26 2006

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Staining and Labeling
Capsulorhexis
Trypan Blue
Scanning Transmission Electron Microscopy
Indocyanine Green
Endothelial Cells
Electron Scanning Microscopy
Corneal Endothelium
DNA Nucleotidylexotransferase
coomassie Brilliant Blue
Poisons
In Situ Nick-End Labeling
Biotin
Cell Death
Swine
Cell Count
Fluorescence
Light

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Staining ability and biocompatibility of brilliant blue G : Preclinical study of brilliant blue G as an adjunct for capsular staining. / Hisatomi, Toshio; Enaida, Hiroshi; Matsumoto, Hiroyoshi; Kagimoto, Tadahisa; Ueno, Akifumi; Hata, Yasuaki; Kubota, Toshiaki; Goto, Yoshinobu; Ishibashi, Tatsuro.

In: Archives of Ophthalmology, Vol. 124, No. 4, 26.04.2006, p. 514-519.

Research output: Contribution to journalArticle

Hisatomi, Toshio ; Enaida, Hiroshi ; Matsumoto, Hiroyoshi ; Kagimoto, Tadahisa ; Ueno, Akifumi ; Hata, Yasuaki ; Kubota, Toshiaki ; Goto, Yoshinobu ; Ishibashi, Tatsuro. / Staining ability and biocompatibility of brilliant blue G : Preclinical study of brilliant blue G as an adjunct for capsular staining. In: Archives of Ophthalmology. 2006 ; Vol. 124, No. 4. pp. 514-519.
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AU - Kagimoto, Tadahisa

AU - Ueno, Akifumi

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