Stalled replication forks: Making ends meet for recognition and stabilization

Hisao Masai, Taku Tanaka, Daisuke Kohda

    Research output: Contribution to journalReview article

    34 Citations (Scopus)

    Abstract

    In bacteria, PriA protein, a conserved DEXH-type DNA helicase, plays a central role in replication restart at stalled replication forks. Its unique DNA-binding property allows it to recognize and stabilize stalled forks and the structures derived from them. Cells must cope with fork stalls caused by various replication stresses to complete replication of the entire genome. Failure of the stalled fork stabilization process and eventual restart could lead to various forms of genomic instability. The low viability of priA null cells indicates a frequent occurrence of fork stall during normal growth that needs to be properly processed. PriA specifically recognizes the 30-terminus of the nascent leading strand or the invading strand in a displacement (D)-loop by the three-prime terminus binding pocket (TT-pocket) present in its unique DNA binding domain. Elucidation of the structural basis for recognition of arrested forks by PriA should provide useful insight into how stalled forks are recognized in eukaryotes.

    Original languageEnglish
    Pages (from-to)687-697
    Number of pages11
    JournalBioEssays
    Volume32
    Issue number8
    DOIs
    Publication statusPublished - Aug 1 2010

    All Science Journal Classification (ASJC) codes

    • Biochemistry, Genetics and Molecular Biology(all)

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