Steady-state bioluminescence of bacterial luciferase using electrochemical regeneration of flavin substrate and its application to inhibitory analysis

Shinya Yamasaki, Shinya Nakashima, Shuto Yamada, Kô Takehara

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The model system for the biological reaction using a bacterial luciferase (BL) was developed and applied to the inhibitory analysis of the hydrophobic molecules for enzymatic reactions. The homemade flow electrochemical luminescence cell was embedded in the BL reaction system to regenerate the reduced form of the flavin mononucleotide, which is one of the substrates of the BL luminescence reaction, and to measure the luminescence intensity. The constant intensity of the continuous BL luminescence was observed using the continuous-flow BL reaction system. The proposed system was successfully applied to the inhibitory reaction of dodecaneamide on the BL luminescence reaction.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalBioelectrochemistry
Volume75
Issue number1
DOIs
Publication statusPublished - Apr 1 2009

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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