Steap4 plays a critical role in osteoclastogenesis in vitro by regulating cellular iron/reactive oxygen species (ROS) levels and cAMP response element-binding protein (CREB) activation

Jian Zhou, Shiqiao Ye, Toshifumi Fujiwara, Stavros C. Manolagas, Haibo Zhao

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Background: Iron uptake through the transferrin-dependent pathway is essential for osteoclast differentiation. Results: Knocking down the expression of Steap4, an endosomal ferrireductase, inhibits osteoclast formation and decreases cellular iron and ROS production. Conclusion: Steap4 regulates cellular iron metabolism during osteoclast differentiation. Significance: This work provides new insights into the molecular mechanisms regulating cellular iron metabolism in osteoclast lineage cells.

Original languageEnglish
Pages (from-to)30064-30074
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
Publication statusPublished - Oct 18 2013
Externally publishedYes

Fingerprint

Cyclic AMP Response Element-Binding Protein
Osteoclasts
Osteogenesis
Reactive Oxygen Species
Iron
Chemical activation
Metabolism
Transferrin
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Steap4 plays a critical role in osteoclastogenesis in vitro by regulating cellular iron/reactive oxygen species (ROS) levels and cAMP response element-binding protein (CREB) activation. / Zhou, Jian; Ye, Shiqiao; Fujiwara, Toshifumi; Manolagas, Stavros C.; Zhao, Haibo.

In: Journal of Biological Chemistry, Vol. 288, No. 42, 18.10.2013, p. 30064-30074.

Research output: Contribution to journalArticle

@article{d6cf68c2b8944938b88529f3beb754fc,
title = "Steap4 plays a critical role in osteoclastogenesis in vitro by regulating cellular iron/reactive oxygen species (ROS) levels and cAMP response element-binding protein (CREB) activation",
abstract = "Background: Iron uptake through the transferrin-dependent pathway is essential for osteoclast differentiation. Results: Knocking down the expression of Steap4, an endosomal ferrireductase, inhibits osteoclast formation and decreases cellular iron and ROS production. Conclusion: Steap4 regulates cellular iron metabolism during osteoclast differentiation. Significance: This work provides new insights into the molecular mechanisms regulating cellular iron metabolism in osteoclast lineage cells.",
author = "Jian Zhou and Shiqiao Ye and Toshifumi Fujiwara and Manolagas, {Stavros C.} and Haibo Zhao",
year = "2013",
month = "10",
day = "18",
doi = "10.1074/jbc.M113.478750",
language = "English",
volume = "288",
pages = "30064--30074",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "42",

}

TY - JOUR

T1 - Steap4 plays a critical role in osteoclastogenesis in vitro by regulating cellular iron/reactive oxygen species (ROS) levels and cAMP response element-binding protein (CREB) activation

AU - Zhou, Jian

AU - Ye, Shiqiao

AU - Fujiwara, Toshifumi

AU - Manolagas, Stavros C.

AU - Zhao, Haibo

PY - 2013/10/18

Y1 - 2013/10/18

N2 - Background: Iron uptake through the transferrin-dependent pathway is essential for osteoclast differentiation. Results: Knocking down the expression of Steap4, an endosomal ferrireductase, inhibits osteoclast formation and decreases cellular iron and ROS production. Conclusion: Steap4 regulates cellular iron metabolism during osteoclast differentiation. Significance: This work provides new insights into the molecular mechanisms regulating cellular iron metabolism in osteoclast lineage cells.

AB - Background: Iron uptake through the transferrin-dependent pathway is essential for osteoclast differentiation. Results: Knocking down the expression of Steap4, an endosomal ferrireductase, inhibits osteoclast formation and decreases cellular iron and ROS production. Conclusion: Steap4 regulates cellular iron metabolism during osteoclast differentiation. Significance: This work provides new insights into the molecular mechanisms regulating cellular iron metabolism in osteoclast lineage cells.

UR - http://www.scopus.com/inward/record.url?scp=84886880324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886880324&partnerID=8YFLogxK

U2 - 10.1074/jbc.M113.478750

DO - 10.1074/jbc.M113.478750

M3 - Article

C2 - 23990467

AN - SCOPUS:84886880324

VL - 288

SP - 30064

EP - 30074

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 42

ER -