Stepwise differentiation from Naïve state pluripotent stem cells to functional primordial germ cells through an epiblast-like state

Katsuhiko Hayashi, Mitinori Saitou

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

A group of pluripotent cells appearing during mammalian embryogenesis is the source for all the cell lineages that compose the embryo proper. In mice, pluripotent cells are first established in the inner cell mass (ICM) of the preimplantation blastocyst. After implantation, the ICM soon transforms into a cup-shaped epithelium, called the postimplantation epiblast. The two types of pluripotent cells, the ICM and postimplantation epiblast cells, are distinct, based on the differences in their gene expression profiles, epigenetic status, and differentiation capacity. During gastrulation, some of the postimplantation epiblast cells adjacent to the extraembryonic ectoderm are specified as primordial germ cells (PGCs), precursors of the germ cell lineage, in response to bone morphogenetic protein 4 (BMP4). Recently, we succeeded in reconstituting epiblast differentiation and PGC specification in vitro using pluripotent stem cells. Here, we describe the culture method of a stepwise differentiation from pluripotent stem cells to functional PGCs.

Original languageEnglish
Title of host publicationEpiblast Stem Cells
Subtitle of host publicationMethods and Protocols
EditorsRamiro Alberio
Pages175-183
Number of pages9
DOIs
Publication statusPublished - Sep 20 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1074
ISSN (Print)1064-3745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Hayashi, K., & Saitou, M. (2013). Stepwise differentiation from Naïve state pluripotent stem cells to functional primordial germ cells through an epiblast-like state. In R. Alberio (Ed.), Epiblast Stem Cells: Methods and Protocols (pp. 175-183). (Methods in Molecular Biology; Vol. 1074). https://doi.org/10.1007/978-1-62703-628-3_13