Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells

Kazuyuki Onodera, Ichiro Takahashi, Yasuyuki Sasano, Jin Wan Bae, Hidetoshi Mitani, Manabu Kagayama, Hideo Mitani

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Biomechanical forces are major epigenetic factors that determine the form and differentiation of skeletal tissues, and may be transduced through cell adhesion to the intracellular biochemical signaling pathway. To test the hypothesis that stepwise stretching is translated to molecular signals during early chondrogenesis, we developed a culture system to study the proliferation and differentiation of chondrocytes. Rat embryonic day-12 limb buds were microdissected and dissociated into cells, which were then micromass cultured on a silicone membrane and maintained for up to 7 days. Stepwise-increased stretching was applied to the silicone membrane, which exerted shearing stress on the cultures on day 4 after the initiation of chondrogenesis. Under stretched conditions, type II collagen expression was significantly inhibited by 44% on day 1 and by 67% on day 2, and this difference in type II collagen reached 80% after 3 days of culture. Accumulation of type II collagen protein and the size of the chondrogenic nodules had decreased by 50% on day 3. On the other hand, expression of the non-chondrogenic marker fibronectin was significantly up-regulated by 1.8-fold on day 3, while the up-regulation of type I collagen was minimal, even by day 3. The down-regulation in the expression of chondrogenic markers was completely recovered when cell-extracellular matrix attachment was inhibited by Gly-Arg-Gly-Asp-Ser-Pro-Lys peptide or by the application of blocking antibodies for α2, α5 or β1 integrins. We conclude that shearing stress generated by stepwise stretching inhibits chondrogenesis through integrins, and propose that signal transduction from biomechanical stimuli may be mediated by cell-extracellular matrix adhesion.

Original languageEnglish
Pages (from-to)45-58
Number of pages14
JournalEuropean Journal of Cell Biology
Volume84
Issue number1
DOIs
Publication statusPublished - Jan 31 2005
Externally publishedYes

Fingerprint

Cell-Matrix Junctions
Limb Buds
Chondrogenesis
Collagen Type II
Integrins
glycyl- arginyl-glycyl-aspartyl-seryl-prolyl-lysine
Silicones
Extracellular Matrix
Blocking Antibodies
Membranes
Chondrocytes
Collagen Type I
Fibronectins
Epigenomics
Cell Adhesion
Signal Transduction
Up-Regulation
Down-Regulation
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells. / Onodera, Kazuyuki; Takahashi, Ichiro; Sasano, Yasuyuki; Bae, Jin Wan; Mitani, Hidetoshi; Kagayama, Manabu; Mitani, Hideo.

In: European Journal of Cell Biology, Vol. 84, No. 1, 31.01.2005, p. 45-58.

Research output: Contribution to journalArticle

Onodera, Kazuyuki ; Takahashi, Ichiro ; Sasano, Yasuyuki ; Bae, Jin Wan ; Mitani, Hidetoshi ; Kagayama, Manabu ; Mitani, Hideo. / Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells. In: European Journal of Cell Biology. 2005 ; Vol. 84, No. 1. pp. 45-58.
@article{5d5aa0ee5a2e452aa7961b24543c933a,
title = "Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells",
abstract = "Biomechanical forces are major epigenetic factors that determine the form and differentiation of skeletal tissues, and may be transduced through cell adhesion to the intracellular biochemical signaling pathway. To test the hypothesis that stepwise stretching is translated to molecular signals during early chondrogenesis, we developed a culture system to study the proliferation and differentiation of chondrocytes. Rat embryonic day-12 limb buds were microdissected and dissociated into cells, which were then micromass cultured on a silicone membrane and maintained for up to 7 days. Stepwise-increased stretching was applied to the silicone membrane, which exerted shearing stress on the cultures on day 4 after the initiation of chondrogenesis. Under stretched conditions, type II collagen expression was significantly inhibited by 44{\%} on day 1 and by 67{\%} on day 2, and this difference in type II collagen reached 80{\%} after 3 days of culture. Accumulation of type II collagen protein and the size of the chondrogenic nodules had decreased by 50{\%} on day 3. On the other hand, expression of the non-chondrogenic marker fibronectin was significantly up-regulated by 1.8-fold on day 3, while the up-regulation of type I collagen was minimal, even by day 3. The down-regulation in the expression of chondrogenic markers was completely recovered when cell-extracellular matrix attachment was inhibited by Gly-Arg-Gly-Asp-Ser-Pro-Lys peptide or by the application of blocking antibodies for α2, α5 or β1 integrins. We conclude that shearing stress generated by stepwise stretching inhibits chondrogenesis through integrins, and propose that signal transduction from biomechanical stimuli may be mediated by cell-extracellular matrix adhesion.",
author = "Kazuyuki Onodera and Ichiro Takahashi and Yasuyuki Sasano and Bae, {Jin Wan} and Hidetoshi Mitani and Manabu Kagayama and Hideo Mitani",
year = "2005",
month = "1",
day = "31",
doi = "10.1016/j.ejcb.2004.09.004",
language = "English",
volume = "84",
pages = "45--58",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "1",

}

TY - JOUR

T1 - Stepwise mechanical stretching inhibits chondrogenesis through cell-matrix adhesion mediated by integrins in embryonic rat limb-bud mesenchymal cells

AU - Onodera, Kazuyuki

AU - Takahashi, Ichiro

AU - Sasano, Yasuyuki

AU - Bae, Jin Wan

AU - Mitani, Hidetoshi

AU - Kagayama, Manabu

AU - Mitani, Hideo

PY - 2005/1/31

Y1 - 2005/1/31

N2 - Biomechanical forces are major epigenetic factors that determine the form and differentiation of skeletal tissues, and may be transduced through cell adhesion to the intracellular biochemical signaling pathway. To test the hypothesis that stepwise stretching is translated to molecular signals during early chondrogenesis, we developed a culture system to study the proliferation and differentiation of chondrocytes. Rat embryonic day-12 limb buds were microdissected and dissociated into cells, which were then micromass cultured on a silicone membrane and maintained for up to 7 days. Stepwise-increased stretching was applied to the silicone membrane, which exerted shearing stress on the cultures on day 4 after the initiation of chondrogenesis. Under stretched conditions, type II collagen expression was significantly inhibited by 44% on day 1 and by 67% on day 2, and this difference in type II collagen reached 80% after 3 days of culture. Accumulation of type II collagen protein and the size of the chondrogenic nodules had decreased by 50% on day 3. On the other hand, expression of the non-chondrogenic marker fibronectin was significantly up-regulated by 1.8-fold on day 3, while the up-regulation of type I collagen was minimal, even by day 3. The down-regulation in the expression of chondrogenic markers was completely recovered when cell-extracellular matrix attachment was inhibited by Gly-Arg-Gly-Asp-Ser-Pro-Lys peptide or by the application of blocking antibodies for α2, α5 or β1 integrins. We conclude that shearing stress generated by stepwise stretching inhibits chondrogenesis through integrins, and propose that signal transduction from biomechanical stimuli may be mediated by cell-extracellular matrix adhesion.

AB - Biomechanical forces are major epigenetic factors that determine the form and differentiation of skeletal tissues, and may be transduced through cell adhesion to the intracellular biochemical signaling pathway. To test the hypothesis that stepwise stretching is translated to molecular signals during early chondrogenesis, we developed a culture system to study the proliferation and differentiation of chondrocytes. Rat embryonic day-12 limb buds were microdissected and dissociated into cells, which were then micromass cultured on a silicone membrane and maintained for up to 7 days. Stepwise-increased stretching was applied to the silicone membrane, which exerted shearing stress on the cultures on day 4 after the initiation of chondrogenesis. Under stretched conditions, type II collagen expression was significantly inhibited by 44% on day 1 and by 67% on day 2, and this difference in type II collagen reached 80% after 3 days of culture. Accumulation of type II collagen protein and the size of the chondrogenic nodules had decreased by 50% on day 3. On the other hand, expression of the non-chondrogenic marker fibronectin was significantly up-regulated by 1.8-fold on day 3, while the up-regulation of type I collagen was minimal, even by day 3. The down-regulation in the expression of chondrogenic markers was completely recovered when cell-extracellular matrix attachment was inhibited by Gly-Arg-Gly-Asp-Ser-Pro-Lys peptide or by the application of blocking antibodies for α2, α5 or β1 integrins. We conclude that shearing stress generated by stepwise stretching inhibits chondrogenesis through integrins, and propose that signal transduction from biomechanical stimuli may be mediated by cell-extracellular matrix adhesion.

UR - http://www.scopus.com/inward/record.url?scp=12544250862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12544250862&partnerID=8YFLogxK

U2 - 10.1016/j.ejcb.2004.09.004

DO - 10.1016/j.ejcb.2004.09.004

M3 - Article

C2 - 15724815

AN - SCOPUS:12544250862

VL - 84

SP - 45

EP - 58

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 1

ER -