Stereoselective esterification of dl-menthol by polyurethane-entrapped lipase in organic solvent

Satoru Koshiro, Kenji Sonomoto, Atsuo Tanaka, Saburo Fukui

Research output: Contribution to journalArticle

90 Citations (Scopus)


Stereospecific esterification of dl-menthol was studied by the use of immobilized lipase in an adequate water-saturated organic solvent system. Lipase from Candida cylindracea immobilized by entrapment with urethane prepolymers and 5-phenylvaleric acid as the acyl donor were chosen based on the stereoselectivity and the yield of l-menthyl ester. Water-saturated cyclohexane or isooctane was found to be the most suitable solvent system. Entrapment significantly enhanced the operational stability of lipase.

Original languageEnglish
Pages (from-to)47-57
Number of pages11
JournalJournal of Biotechnology
Issue number1
Publication statusPublished - Feb 1985


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this