The sterilizing effect of supercritical carbon dioxide (SC-C02) is described as compared with aseous C02(G-C02) and liquid C02(L-C02). Baker’s yeast, Escherichia coli, Staphylococcus aureus and conidia of Aspergillus niger were sterilized by treating with SC-C02 at 200 atm and 35°C when the water content of each microorganism was 70-90%. However, dry cells with a water content of 2 - 10% could not be sterilized under the same conditions. No sterilizing effect from SC- C02 on endospores of Bacillus subtilis and B. stearothermophilus was observed. G-C02 and L-C02produced no sterilizing effect against both wet and dry baker’s yeast cells, while wet E. coli cells were sterilized with G-C02. Although dry conidia of A. niger could not be sterilized with SC-C02alone, the addition of ethanol or acetic acid to SC-C02 made it possible to sterilize these. Without any decrease in their enzymatic activities, E. preparations were sterilized with SC-C02.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)