Steroid 9α-hydroxylation during testosterone degradation by resting Rhodococcus equi cells

Yong Ung Kim, Jaehong Han, Sup Lee Sang, Kuniyoshi Shimizu, Yuji Tsutsumi, Ryuichiro Kondo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The conversion pathway of testosterone to androst-4-ene-3,17-dione and 9α-hydroxy androstane metabolites, 9α-hydroxyandrost-4-ene-3,17- dione and 9α,17β-dihydroxyandrost-4-en-3-one was proposed for the ring degradation in steroids by a minimal liquid medium (NMMP)-dispersed Rhodococcus equi ATCC 14887. The microorganism produced 9α-hydroxy androstane metabolites from testosterone at high conversion ratio without the addition of ring degradation inhibitory agents. Several NMMP-based media showed the similar effect on the microbial transformation, in which the respective molar yields of 9α-hydroxyandrost-4-ene-3,17-dione and 9α,17β-dihydroxyandrost-4-en-3-one were approx. 3 to 47% and approx. 3 to 11%, respectively, whereas nutrient broth, a rich medium, basically showed no accumulation. On the basis of this evidence, magnesium sulfate and casamino acids among the components of NMMP were found to compromise the determinant for the production of the 9α-hydroxy androstane metabolites without appreciable decomposition of the steroid ring system.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalArchiv der Pharmazie
Volume340
Issue number4
DOIs
Publication statusPublished - Apr 1 2007

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery

Cite this