Steroid hormones and their synthetic pathways in the vitellogenic ovarian follicles of yellowtail, Seriola quinqueradiata

M. Anisur Rahman, Kohei Ohta, Hisashi Chuda, Akihiko Yamaguchi, Michiya Matsuyama

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2 Citations (Scopus)

Abstract

In the present study, we clarified the steroid hormones produced and their synthetic pathways, particularly focusing the estradiol-17β (E2) synthesis, in the vitellogenic ovarian follicles to provide information on the endocrine control of vitellogenesis in yellowtail, Seriola quinqueradiata. Intact vitellogenic ovarian follicles were isolated and incubated with radioactive [3H]pregnenolone and [14C]androstenedione and steroid metabolites were identified by thin layer chromatography (TLC) followed by recrystallization to constant specific activity. Results obtained clearly indicated that testosterone (T) is the substrate precursor of E2 synthesis. In the vitellogenic ovarian follicles, the steroid metabolites produced and major pathway followed were pregnenolone, 17-hydroxypregnenolone, dehydroepiandrosterone, androstenedione (AD), T and E2. AD, T and E2 were also present in the serum of fish during vitellogenesis, and serum level of T was the highest (3.7ng/ml), followed by E2 (2.3ng/ml). Thus, this study demonstrated the complete steroidogenic pathway of E2 synthesis in the ovarian follicles of yellowtail, and revealed that T is the major precursor of E2.

Original languageEnglish
Pages (from-to)311-319
Number of pages9
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume46
Issue number2
Publication statusPublished - Oct 2002

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Seriola quinqueradiata
Ovarian Follicle
ovarian follicles
steroid hormones
androstenedione
Androstenedione
Steroids
Hormones
Vitellogenesis
pregnenolone
Pregnenolone
vitellogenesis
steroids
synthesis
17-alpha-Hydroxypregnenolone
metabolites
prasterone
Dehydroepiandrosterone
Thin Layer Chromatography
Serum

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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title = "Steroid hormones and their synthetic pathways in the vitellogenic ovarian follicles of yellowtail, Seriola quinqueradiata",
abstract = "In the present study, we clarified the steroid hormones produced and their synthetic pathways, particularly focusing the estradiol-17β (E2) synthesis, in the vitellogenic ovarian follicles to provide information on the endocrine control of vitellogenesis in yellowtail, Seriola quinqueradiata. Intact vitellogenic ovarian follicles were isolated and incubated with radioactive [3H]pregnenolone and [14C]androstenedione and steroid metabolites were identified by thin layer chromatography (TLC) followed by recrystallization to constant specific activity. Results obtained clearly indicated that testosterone (T) is the substrate precursor of E2 synthesis. In the vitellogenic ovarian follicles, the steroid metabolites produced and major pathway followed were pregnenolone, 17-hydroxypregnenolone, dehydroepiandrosterone, androstenedione (AD), T and E2. AD, T and E2 were also present in the serum of fish during vitellogenesis, and serum level of T was the highest (3.7ng/ml), followed by E2 (2.3ng/ml). Thus, this study demonstrated the complete steroidogenic pathway of E2 synthesis in the ovarian follicles of yellowtail, and revealed that T is the major precursor of E2.",
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T1 - Steroid hormones and their synthetic pathways in the vitellogenic ovarian follicles of yellowtail, Seriola quinqueradiata

AU - Rahman, M. Anisur

AU - Ohta, Kohei

AU - Chuda, Hisashi

AU - Yamaguchi, Akihiko

AU - Matsuyama, Michiya

PY - 2002/10

Y1 - 2002/10

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AB - In the present study, we clarified the steroid hormones produced and their synthetic pathways, particularly focusing the estradiol-17β (E2) synthesis, in the vitellogenic ovarian follicles to provide information on the endocrine control of vitellogenesis in yellowtail, Seriola quinqueradiata. Intact vitellogenic ovarian follicles were isolated and incubated with radioactive [3H]pregnenolone and [14C]androstenedione and steroid metabolites were identified by thin layer chromatography (TLC) followed by recrystallization to constant specific activity. Results obtained clearly indicated that testosterone (T) is the substrate precursor of E2 synthesis. In the vitellogenic ovarian follicles, the steroid metabolites produced and major pathway followed were pregnenolone, 17-hydroxypregnenolone, dehydroepiandrosterone, androstenedione (AD), T and E2. AD, T and E2 were also present in the serum of fish during vitellogenesis, and serum level of T was the highest (3.7ng/ml), followed by E2 (2.3ng/ml). Thus, this study demonstrated the complete steroidogenic pathway of E2 synthesis in the ovarian follicles of yellowtail, and revealed that T is the major precursor of E2.

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