Abstract
Background: The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.Methods: Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.Results: Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.Conclusions: Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.
| Original language | English |
|---|---|
| Article number | 71 |
| Journal | Reproductive Biology and Endocrinology |
| Volume | 10 |
| DOIs | |
| Publication status | Published - Sep 5 2012 |
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All Science Journal Classification (ASJC) codes
- Reproductive Medicine
- Endocrinology
- Obstetrics and Gynaecology
- Developmental Biology
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Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus. / Ohga, Hirofumi; Kaneko, Kensuke; Shimizu, Akio; Kitano, Hajime; Selvaraj, Sethu; Nyuji, Mitsuo; Adachi, Hayato; Yamaguchi, Akihiko; Matsuyama, Michiya.
In: Reproductive Biology and Endocrinology, Vol. 10, 71, 05.09.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus
AU - Ohga, Hirofumi
AU - Kaneko, Kensuke
AU - Shimizu, Akio
AU - Kitano, Hajime
AU - Selvaraj, Sethu
AU - Nyuji, Mitsuo
AU - Adachi, Hayato
AU - Yamaguchi, Akihiko
AU - Matsuyama, Michiya
PY - 2012/9/5
Y1 - 2012/9/5
N2 - Background: The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.Methods: Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.Results: Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.Conclusions: Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.
AB - Background: The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.Methods: Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.Results: Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.Conclusions: Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.
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UR - http://www.scopus.com/inward/citedby.url?scp=84865644255&partnerID=8YFLogxK
U2 - 10.1186/1477-7827-10-71
DO - 10.1186/1477-7827-10-71
M3 - Article
C2 - 22950645
AN - SCOPUS:84865644255
VL - 10
JO - Reproductive Biology and Endocrinology
JF - Reproductive Biology and Endocrinology
SN - 1477-7827
M1 - 71
ER -