Sterol effect on interaction between amphidinol 3 and liposomal membrane as evidenced by surface plasmon resonance

Respati T. Swasono, Ryota Mouri, Nagy Morsy, Nobuaki Matsumori, Tohru Oishi, Michio Murata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The affinity of amphidinol 3 (AM3) to phospholipid membranes in the presence and absence of sterol was examined by surface plasmon resonance (SPR) experiments. The results showed that AM3 has 1000 and 5300 times higher affinity for cholesterol- and ergosterol-containing liposomes, respectively, than those without sterol. The two-state reaction model well reproduced the sensor grams, which indicated that the interaction is composed of two steps, which correspond to binding to the membrane and internalization to form stable complexes.

Original languageEnglish
Pages (from-to)2215-2218
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume20
Issue number7
DOIs
Publication statusPublished - Apr 1 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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