Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-β-glucosidase identified in fungi

Takashi Watanabe, Tomoharu Ito, Hatsumi M. Goda, Yohei Ishibashi, Tomofumi Miyamoto, Kazutaka Ikeda, Ryo Taguchi, Nozomu Okino, Makoto Ito

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Endoglycoceramidase-related protein 1 (EGCrP1) is a glucocerebrosidase in Cryptococcus neoformans; however, the functions of its paralogue, EGCrP2, remain unknown. Results: EGCrP2, but not EGCrP1, hydrolyzed steryl-β-glucosides. Disruption of egcrp2 accumulated ergosteryl-3β-glucoside in C. neoformans. Conclusion: EGCrP2 degraded steryl-β-glucosides both in vivo and in vitro. Significance: EGCrP2 is a missing link in steryl-β-glucoside metabolism in fungi. In addition, EGCrP2 is a potential target for antifungal drugs.

Original languageEnglish
Pages (from-to)1005-1019
Number of pages15
JournalJournal of Biological Chemistry
Volume290
Issue number2
DOIs
Publication statusPublished - Sep 9 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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