Background: Endoglycoceramidase-related protein 1 (EGCrP1) is a glucocerebrosidase in Cryptococcus neoformans; however, the functions of its paralogue, EGCrP2, remain unknown. Results: EGCrP2, but not EGCrP1, hydrolyzed steryl-β-glucosides. Disruption of egcrp2 accumulated ergosteryl-3β-glucoside in C. neoformans. Conclusion: EGCrP2 degraded steryl-β-glucosides both in vivo and in vitro. Significance: EGCrP2 is a missing link in steryl-β-glucoside metabolism in fungi. In addition, EGCrP2 is a potential target for antifungal drugs.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology