Stiffness-index map based on single cell-spheroid analysis using robot integrated microfluidic chiip

Keitaro Ito, Shinya Sakuma, Masaki Kimura, Takanori Takebe, Makoto Kaneko, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a method which can evaluate stiffness of cell-spheroids, and show the measurement results of the stiffness changes with respect to the culture time. To obtain the stiffness-index map, where the map shows the relationship between the size and stiffness of cell-spheroids, we applied on-chip measurement method using a robot integrated microfluidic chip (robochip). The robochip has a microchannel and a pair of on-chip probes which contains a force sensor. By measuring the stiffness in microfluidic channel, we can achieve a stable and high-throughput evaluation of mechanical characteristics. The stiffness of the single spheroid is evaluated from the reactive force when the target spheroid is deformed by the on-chip probes. The stiffness-index of cell-spheroid is proposed, and the evaluation results of the stiffness index shows that the index increased along the culture days. The proposed system will provide us a new index for the cell-quality evaluation.

Original languageEnglish
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages157-160
Number of pages4
ISBN (Electronic)9781509019731
DOIs
Publication statusPublished - Feb 26 2016
Externally publishedYes
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: Jan 24 2016Jan 28 2016

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2016-February
ISSN (Print)1084-6999

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
CountryChina
CityShanghai
Period1/24/161/28/16

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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