Stimulation by 2-Mercaptoethanol and Alcohols of Protease in Antarctic Krill, Euphausia superba, at Low Temperatures

Koji Yamada, Hiroki Murakami, Hirofumi Tachibana, Yumi Watanabe, Hirohisa Omura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We fractionated the homogenate of Antarctic krill, Euphausia superba, with a Sephacryl S-400 column and obtained two protease fractions, A (M.W. 50 K) and B (M.W. 30 K). Both fractions digested preferentially the peptide bond adjacent to arginine, and also digested peptide bonds adjacent to lysine and leucine. Their optimum pH was 9.0 and optimum temperature was 37°C in the absence of 2-mercaptoethanol. In the presence of 4.4% 2-mercaptoethanol, the activity of fraction A was stimulated below 30°C, but inhibited at higher temperatures. This led to the shift of optimum temperature from 37°C to 20°C. Some of alcohols also strongly stimulated the krill protease at 20°C.

Original languageEnglish
Pages (from-to)3363-3368
Number of pages6
JournalAgricultural and Biological Chemistry
Volume51
Issue number12
DOIs
Publication statusPublished - Jan 1 1987

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Euphausiacea
Euphausia superba
Mercaptoethanol
Peptide Hydrolases
proteinases
alcohols
Alcohols
Temperature
peptides
Peptides
temperature
krill
Leucine
Lysine
leucine
arginine
Arginine
lysine
beta-mercaptoethanol

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Stimulation by 2-Mercaptoethanol and Alcohols of Protease in Antarctic Krill, Euphausia superba, at Low Temperatures. / Yamada, Koji; Murakami, Hiroki; Tachibana, Hirofumi; Watanabe, Yumi; Omura, Hirohisa.

In: Agricultural and Biological Chemistry, Vol. 51, No. 12, 01.01.1987, p. 3363-3368.

Research output: Contribution to journalArticle

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