Stimulation of Sendai virus C′ protein synthesis by cycloheximide

Kailash C. Gupta, Etsuro Ono

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The polycistronic Sendai virus P/C mRNA is translated into five proteins (P, C′, C, Y1 and Y2) from distinct start sites in virus-infected cells. The translation mechanism(s) of these proteins from two overlapping open reading frames in the P/C mRNA are poorly understood [Gupta, Ono and Xu (1996) Biochemistry 35, 1223-1231]. While investigating the initiation mechanism of C′ from an ACG start site, we found that C′ synthesis was resistant to inhibitors of peptide chain elongation such as cycloheximide (CHX) and anisomycin, but not to pactamycin (an inhibitor of chain initiation) or puromycin (a peptide chain terminator). Moreover, low levels (less than 30 μg/ml) of CHX significantly stimulated C′ synthesis. Whereas C′ synthesis was stimulated, synthesis of the P and C proteins, which are translated from the same mRNA, decreased by more than 95%. Stimulation of C′ synthesis by CHX is not related to its initiation at an ACG codon. Mutation of ACG to alternative start sites had no effect on the CHX-stimulated C' synthesis. Similarly, C' synthesis was preferentially stimulated when Sendai virus-infected cells were exposed to hypotonie growth medium. These results suggest that the P/C mRNA may exist in at least two reversible conformations: whereas one conformation allows synthesis of the P and C proteins, the alternative conformation allows synthesis of the C′ protein. It might be that low concentrations of CHX somehow increase the alternative conformation, which increases C′ synthesis. The C′ protein synthesis is reminiscent of the synthesis of stress-related proteins. Perhaps Sendai virus has evolved a novel mechanism to express both non-stress-related and stress-related proteins from the same mRNA.

Original languageEnglish
Pages (from-to)811-818
Number of pages8
JournalBiochemical Journal
Volume321
Issue number3
DOIs
Publication statusPublished - Feb 1 1997

Fingerprint

Cycloheximide
Protein C
Sendai virus
Messenger RNA
Heat-Shock Proteins
Pactamycin
Viruses
Conformations
Anisomycin
Puromycin
Peptides
Protein Conformation
Codon
Biochemistry
Open Reading Frames
Sendai virus nonstructural C protein
Proteins
Mutation
Growth
Elongation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Stimulation of Sendai virus C′ protein synthesis by cycloheximide. / Gupta, Kailash C.; Ono, Etsuro.

In: Biochemical Journal, Vol. 321, No. 3, 01.02.1997, p. 811-818.

Research output: Contribution to journalArticle

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