TY - JOUR
T1 - Stimulatory activity on prostacyclin production decreases in sera from streptozotocin-induced diabetic rats
AU - Inoguchi, T.
AU - Umeda, F.
AU - Watanabe, J.
AU - Ibayashi, H.
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (Grant No. 462180150431) of the Ministry of Education, Science and Culture, Japan.
PY - 1987
Y1 - 1987
N2 - We recently reported that serum stimulatory activity on prostacyclin (PGI2) production by cultured bovine aortic endothelial cells decreased in noninsulin-dependent diabetic patients. In the present study, this activity was compared in streptozotocin-induced (STZ) diabetic rats and controls. Platelet-poor plasma-derived serum (PDS) from Wistar male rats stimulated 6-keto-PGF1α production (a stable metabolite of PGI2) by cultured bovine aortic endothelial cells, rat lung fibroblasts, and rat aortic rings in a time- and dose-dependent manner. Namely, PDS from rats has a stimulatory activity on PGI2 production (PGI2 stimulatory activity; PSA). Furthermore, PSA in PDS from STZ diabetic rats (n = 12) significantly decreased as compared with that from control rats (n = 10) using three types of in vitro systems. The reduction in PDS-stimulated PGI2 production by the vascular wall may lead to platelet hyperaggregation and thrombus formation in diabetics, which is considered to be involved in the pathogenesis of diabetic macro- or microangiopathy.
AB - We recently reported that serum stimulatory activity on prostacyclin (PGI2) production by cultured bovine aortic endothelial cells decreased in noninsulin-dependent diabetic patients. In the present study, this activity was compared in streptozotocin-induced (STZ) diabetic rats and controls. Platelet-poor plasma-derived serum (PDS) from Wistar male rats stimulated 6-keto-PGF1α production (a stable metabolite of PGI2) by cultured bovine aortic endothelial cells, rat lung fibroblasts, and rat aortic rings in a time- and dose-dependent manner. Namely, PDS from rats has a stimulatory activity on PGI2 production (PGI2 stimulatory activity; PSA). Furthermore, PSA in PDS from STZ diabetic rats (n = 12) significantly decreased as compared with that from control rats (n = 10) using three types of in vitro systems. The reduction in PDS-stimulated PGI2 production by the vascular wall may lead to platelet hyperaggregation and thrombus formation in diabetics, which is considered to be involved in the pathogenesis of diabetic macro- or microangiopathy.
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U2 - 10.1016/S0168-8227(87)80047-5
DO - 10.1016/S0168-8227(87)80047-5
M3 - Article
C2 - 3311677
AN - SCOPUS:0023227906
SN - 0168-8227
VL - 3
SP - 243
EP - 248
JO - Diabetes Research and Clinical Practice
JF - Diabetes Research and Clinical Practice
IS - 5
ER -
