Here we describe a novel strategy of liposome aggregation employing DNA-surfactant, in which the aggregation of liposomes can be reversibly controlled by enzyme stimuli and temperature. Single-stranded oligonucleotides (24-mer) were bound to oleic acid to produce DNA-surfactant. The DNA surfactant was mixed with POPC liposomes to prepare DNA-tethered liposomes. Mixing of the DNA-tagged liposomes with ones bearing complementary DNA strands starts the aggregation of liposomes. The liposomes were linked with each other via duplex DNA. We found that the liposome aggregation was influenced by temperature, salt concentration and restriction enzyme.
|Number of pages||2|
|Publication status||Published - Dec 1 2005|
|Event||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
Duration: Sep 20 2005 → Sep 22 2005
|Other||54th SPSJ Symposium on Macromolecules|
|Period||9/20/05 → 9/22/05|
All Science Journal Classification (ASJC) codes