Stochastic imprinting in the progeny of Dnmt3L-/- females

Philippe Arnaud, Kenichiro Hata, Masahiro Kaneda, En Li, Hiroyuki Sasaki, Robert Feil, Gavin Kelsey

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The cis-acting regulatory sequences of imprinted genes are subject to germline-specific epigenetic modifications, the imprints, so that this class of genes is exclusively expressed from either the paternal or maternal allele in offspring. How genes are differentially marked in the germlines remains largely to be elucidated. Although the exact nature of the mark is not fully known, DNA methylation [at differentially methylated regions (DMRs)] appears to be a major, functional component. Recent data in mice indicate that Dnmt3a, an enzyme with de novo DNA methyltransferase activity, and the related protein Dnmt3L are required for methylation of imprinted loci in germ cells. Maternal methylation imprints, in particular, are strictly dependent on the presence of Dnmt3L. Here, we show that, unexpectedly, methylation imprints can be present in some progeny of Dnmt3L-/- females. This incomplete penetrance of the effect of Dnmt3L deficiency in oocytes is neither embryo nor locus specific, but stochastic. We establish that, when it occurs, methylation is present in both embryo and extra-embryonic tissues and results in a functional imprint. This suggests that this maternal methylation is inherited, directly or indirectly, from the gamete. Our results indicate that in the absence of Dnmt3L, factors such as Dnmt3a and possibly others can act alone to mark individual DMRs. However, establishment of appropriate maternal imprints at all loci does require a combination of all factors. This observation can provide a basis to understand mechanisms involved in some sporadic cases of imprinting-related diseases and polymorphic imprinting in human.

Original languageEnglish
Pages (from-to)589-598
Number of pages10
JournalHuman Molecular Genetics
Volume15
Issue number4
DOIs
Publication statusPublished - Feb 15 2006

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Methylation
Mothers
Germ Cells
Embryonic Structures
Genes
Penetrance
Methyltransferases
DNA Methylation
Epigenomics
Oocytes
Alleles
DNA
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Arnaud, P., Hata, K., Kaneda, M., Li, E., Sasaki, H., Feil, R., & Kelsey, G. (2006). Stochastic imprinting in the progeny of Dnmt3L-/- females. Human Molecular Genetics, 15(4), 589-598. https://doi.org/10.1093/hmg/ddi475

Stochastic imprinting in the progeny of Dnmt3L-/- females. / Arnaud, Philippe; Hata, Kenichiro; Kaneda, Masahiro; Li, En; Sasaki, Hiroyuki; Feil, Robert; Kelsey, Gavin.

In: Human Molecular Genetics, Vol. 15, No. 4, 15.02.2006, p. 589-598.

Research output: Contribution to journalArticle

Arnaud, P, Hata, K, Kaneda, M, Li, E, Sasaki, H, Feil, R & Kelsey, G 2006, 'Stochastic imprinting in the progeny of Dnmt3L-/- females', Human Molecular Genetics, vol. 15, no. 4, pp. 589-598. https://doi.org/10.1093/hmg/ddi475
Arnaud, Philippe ; Hata, Kenichiro ; Kaneda, Masahiro ; Li, En ; Sasaki, Hiroyuki ; Feil, Robert ; Kelsey, Gavin. / Stochastic imprinting in the progeny of Dnmt3L-/- females. In: Human Molecular Genetics. 2006 ; Vol. 15, No. 4. pp. 589-598.
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