Abstract
Confocal fluorescence microscopy has been seldom applied to air-liquid interfaces due to technical difficulties. Satellite lines of an excitation laser beam can be used as an inherent reference for a confocal microscope to align and calibrate the setup. This strategy is especially useful and important to a liquid surface, and makes it possible to observe very weak fluorescence without time-consuming alignment procedures.
Original language | English |
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Pages (from-to) | 159-166 |
Number of pages | 8 |
Journal | Instrumentation Science and Technology |
Volume | 27 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1999 |
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)
- Instrumentation
- Environmental Science(all)