Streptomyces atp nucleotide 3′-pyrophosphokinase–gene cloning and sequence analysis

Toshio Higuchi, Takeshi Mikuniya, Kazutoyo Osoegawa, Satoshi Ezaki, Hiroshi Sumichika, Yoshiharu Mizui, Toshiharu Shoji, Kenji Kishihara, Shigeru Muta, Satoru Kuhara, Jim Ichiro Mukai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Streptomyces ATP nucleotide 3′-pyrophosphokinase is an extracellular enzyme that transfers 5′-β,γ-pyrophosphoryl groups of ATP to a variety of nucleotides at the 3′-OH site. The enzyme gene was cloned from partially Sau3AI-digested chromosomal DNA of S. morookaensis in S. lividans TK24/pIJ699 and then in E. coli JM83/pUC12. Some transformants produced the active enzyme. The gene was sequenced by the dideoxynucleotide termination procedure. Its GC content was 72%. Its putative promoter regions, showing little homology to that of the Streptomyces consensus type, were pointed out. No sequence homology was found between the pyrophosphokinase and any other known genes including those of the most mechanistically similar bacterial stringent factor and related proteins. Northern hybridization analysis showed that the gene is constitutionally polycistronic and expressed under transcriptional control. Nuclease S1 mapping indicated that the gene transcription starts from its translation initiation site.

Original languageEnglish
Pages (from-to)2182-2187
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume58
Issue number12
DOIs
Publication statusPublished - Jan 1 1994

Fingerprint

Cloning
Streptomyces
Sequence Analysis
Organism Cloning
Nucleotides
Genes
Diphosphotransferases
nucleotide pyrophosphokinase
GTP Pyrophosphokinase
Enzymes
Dideoxynucleotides
Adenosine Triphosphate
Chromosome Mapping
Base Composition
Sequence Homology
Genetic Promoter Regions
Transcription
Escherichia coli
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Higuchi, T., Mikuniya, T., Osoegawa, K., Ezaki, S., Sumichika, H., Mizui, Y., ... Mukai, J. I. (1994). Streptomyces atp nucleotide 3′-pyrophosphokinase–gene cloning and sequence analysis. Bioscience, Biotechnology and Biochemistry, 58(12), 2182-2187. https://doi.org/10.1080/bbb.58.2182

Streptomyces atp nucleotide 3′-pyrophosphokinase–gene cloning and sequence analysis. / Higuchi, Toshio; Mikuniya, Takeshi; Osoegawa, Kazutoyo; Ezaki, Satoshi; Sumichika, Hiroshi; Mizui, Yoshiharu; Shoji, Toshiharu; Kishihara, Kenji; Muta, Shigeru; Kuhara, Satoru; Mukai, Jim Ichiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 58, No. 12, 01.01.1994, p. 2182-2187.

Research output: Contribution to journalArticle

Higuchi, T, Mikuniya, T, Osoegawa, K, Ezaki, S, Sumichika, H, Mizui, Y, Shoji, T, Kishihara, K, Muta, S, Kuhara, S & Mukai, JI 1994, 'Streptomyces atp nucleotide 3′-pyrophosphokinase–gene cloning and sequence analysis', Bioscience, Biotechnology and Biochemistry, vol. 58, no. 12, pp. 2182-2187. https://doi.org/10.1080/bbb.58.2182
Higuchi, Toshio ; Mikuniya, Takeshi ; Osoegawa, Kazutoyo ; Ezaki, Satoshi ; Sumichika, Hiroshi ; Mizui, Yoshiharu ; Shoji, Toshiharu ; Kishihara, Kenji ; Muta, Shigeru ; Kuhara, Satoru ; Mukai, Jim Ichiro. / Streptomyces atp nucleotide 3′-pyrophosphokinase–gene cloning and sequence analysis. In: Bioscience, Biotechnology and Biochemistry. 1994 ; Vol. 58, No. 12. pp. 2182-2187.
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