Streptomyces nucleotide 3′-pyrophosphokinase. Synthesis of deoxynucleotide-3′-pyrophosphates and 3′-pyrophosphoryl→3′-OH transfer activity

Jun Ichiro Mukai, Abdur Razzaque, Toshio Kukita, Sawao Murao, Toyokazu Nishino

Research output: Contribution to journalArticle

Abstract

NucleoCide-3′-pyrophosphoklnase(ATP:nucleoside-S’-phosphate pyrophosphotransferase, E.C.2.7.6.4) of Streptomyces adephospholyticus has been shown to transfer 5′-ß,γ-pyrophosphoryl group of either ATP, dATP or adenosine-5′- tri-3′-diphosphate(pppApp) to 3′-slte of a variety of nucleotldlc substances including both purine and pyrimidine ribomononucleoside-5′-phosphates, short oligoribonucleotides, tRNA and 5′-dl- or triphosphonucleosidic sugars and their derivatives such as GDP-glucose, UDP-glucose, CDP-choline, NAD, FAD and AG-5′-ppp-5′ - Aeb, a cap nucleotide at the 5′-terminus of eucaryote raRNAs. The present paper reports two newly found facts of this enzyme: 1) deoxyATP(pppdA) is active not only as a donor but also as an acceptor of the pyrophosphoryl group giving rise to its 3′-pyrophosphoryl derivative, pppdApp. pppdGpp could also be prepared using dGTP as the acceptor. 2) the enzyme catalyses both 5′- P, y-pyrophosphoryl-β3′-OH kinase reaction(irreversible) and 3′-a,ß-pyrophos- phoryl↔3′-OH transfer reaction(reversible).

Original languageEnglish
Pages (from-to)S451-s456
JournalNucleic acids research
Volume1
DOIs
Publication statusPublished - Jan 1 1978

All Science Journal Classification (ASJC) codes

  • Genetics

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