Stress-induced Changes in the Biogenic Amine Levels and Larval Growth of Tribolium Castaneum Herbst

Takeshi Nagano, Akinori Hirashima, Morifusa Eto

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Such stress factors as mechanical (vibration), thermal (unfavorable temperature), optical (light), and starvation reduced the larval growth of the red flour beetle (Tribolium castaneum Herbst). Various biogenic amines, including octopamine (OA), dopamine (DA), serotonin (5-HT), epinephrine (E), norepinephrine (NE), their precursors, and metabolites, in whole-body T. castaneum were measured by high-performance liquid chromatography coupled with electrochemical detection (ECD). Tyrosine occurred in the highest concentration, followed by OA, tryptophan, and 3,4-dihydroxymandelic acid. The amount of OA was much higher than that of tyramine (a precursor of OA in the biosynthetic pathway) and of synephrine (N-methyl OA). DA, 5-HT, E, NE, and their related substances occurred in extremely low quantities compared with OA. Insects were stressed by vibrating at 1,10,100, or 1000 Hz, optically under a 24-h light (15 W, 50 Hz) photoperiod, thermally by changing the incubation temperature from an initial value of 30°C, or by starvation, which resulted in dramatic changes of levels of biogenic amines, including OA.

Original languageEnglish
Pages (from-to)2085-2089
Number of pages5
JournalBioscience, biotechnology, and biochemistry
Volume57
Issue number12
DOIs
Publication statusPublished - Jan 1 1993

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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