We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and a silica bead with a radius of ∼1 μm as an indenter. The decay of loading force was clearly observed in NIH3T3 cells at a small initial loading force of ∼0.4 nN and was well fitted to the stretched exponential function rather than to a single exponential function. The stretching exponent parameter was ∼0.5 for both indenters, indicating that the stress relaxation observed in NIH3T3 cells consisted of multiple relaxation processes. The time-domain AFM technique described in this report allows us to measure directly the relaxation process of living cells in a range from milliseconds to seconds.
|Number of pages||4|
|Journal||Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers|
|Issue number||8 B|
|Publication status||Published - Aug 23 2007|
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)