Stretching of long DNA molecules in the microvortex induced by laser and ac electric field

Michihiko Nakano, Hirofumi Kurita, Jun Komatsu, Akira Mizuno, Shinji Katsura

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A microvortex is generated around an infrared laser focus where an intense ac electric field is applied. The authors used this optoelectrostatic microvortex for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the optoelectrostatic microvortex, they were stretched around the laser focus. In addition, especially for longer T4 DNA molecules, it was possible to keep it in stretching form for more than 30 s. Using this method, length of DNA molecules can be measured without fixing to a substrate. This method can be applied to DNA molecules longer than about 10 μm.

Original languageEnglish
Article number133901
JournalApplied Physics Letters
Volume89
Issue number13
DOIs
Publication statusPublished - 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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