Stretching of long DNA molecules in the microvortex induced by laser and ac electric field

Michihiko Nakano; Hirofumi Kurita; Jun Komatsu; Akira Mizuno; Shinji Katsura

doi:10.1063/1.2357885

Stretching of long DNA molecules in the microvortex induced by laser and ac electric field

Michihiko Nakano, Hirofumi Kurita, Jun Komatsu, Akira Mizuno, Shinji Katsura

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A microvortex is generated around an infrared laser focus where an intense ac electric field is applied. The authors used this optoelectrostatic microvortex for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the optoelectrostatic microvortex, they were stretched around the laser focus. In addition, especially for longer T4 DNA molecules, it was possible to keep it in stretching form for more than 30 s. Using this method, length of DNA molecules can be measured without fixing to a substrate. This method can be applied to DNA molecules longer than about 10 μm.

Original language	English
Article number	133901
Journal	Applied Physics Letters
Volume	89
Issue number	13
DOIs	https://doi.org/10.1063/1.2357885
Publication status	Published - 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.2357885

Cite this

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title = "Stretching of long DNA molecules in the microvortex induced by laser and ac electric field",

abstract = "A microvortex is generated around an infrared laser focus where an intense ac electric field is applied. The authors used this optoelectrostatic microvortex for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the optoelectrostatic microvortex, they were stretched around the laser focus. In addition, especially for longer T4 DNA molecules, it was possible to keep it in stretching form for more than 30 s. Using this method, length of DNA molecules can be measured without fixing to a substrate. This method can be applied to DNA molecules longer than about 10 μm.",

author = "Michihiko Nakano and Hirofumi Kurita and Jun Komatsu and Akira Mizuno and Shinji Katsura",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 15360440), Japan Society for the Promotion of Science (JSPS) Research Fellowships for Young Scientists (No. 08351), and 21st century COE program, “Ecological Engineering for Homeostatic Activities,” from the Ministry of Education, Culture, Sports, Science and Technology.",

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AU - Nakano, Michihiko

AU - Kurita, Hirofumi

AU - Komatsu, Jun

AU - Mizuno, Akira

AU - Katsura, Shinji

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 15360440), Japan Society for the Promotion of Science (JSPS) Research Fellowships for Young Scientists (No. 08351), and 21st century COE program, “Ecological Engineering for Homeostatic Activities,” from the Ministry of Education, Culture, Sports, Science and Technology.

PY - 2006

Y1 - 2006

N2 - A microvortex is generated around an infrared laser focus where an intense ac electric field is applied. The authors used this optoelectrostatic microvortex for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the optoelectrostatic microvortex, they were stretched around the laser focus. In addition, especially for longer T4 DNA molecules, it was possible to keep it in stretching form for more than 30 s. Using this method, length of DNA molecules can be measured without fixing to a substrate. This method can be applied to DNA molecules longer than about 10 μm.

AB - A microvortex is generated around an infrared laser focus where an intense ac electric field is applied. The authors used this optoelectrostatic microvortex for stretching individual long DNAs. When λ-or T4-phage DNA molecules were introduced into the optoelectrostatic microvortex, they were stretched around the laser focus. In addition, especially for longer T4 DNA molecules, it was possible to keep it in stretching form for more than 30 s. Using this method, length of DNA molecules can be measured without fixing to a substrate. This method can be applied to DNA molecules longer than about 10 μm.

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Stretching of long DNA molecules in the microvortex induced by laser and ac electric field

Abstract

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