Abstract
Stromal cell-derived factor-1 (SDF-1) causes chemotaxis of CXCR4-expressing bone marrow-derived cells. SDF-1 is involved in the pathogenesis of various vascular diseases, including those of the eye. However, the role of SDF-1 in neuronal diseases is not completely understood. Here, we show higher SDF-1 levels in the vitreous humor of patients with retinal detachment (RD) compared with normal patients. SDF-1 correlated positively with the duration as well as the extent of RD. Furthermore, SDF-1 correlated significantly with levels of interleukin-6 and interleukin-8, but not with vascular endothelial growth factor. Western blot analysis results showed significant SDF-1 up-regulation in detached rat retinas compared with normal animals. Immunohistochemistry data showed that SDF-1 was co-localized with the glial cells of the detached retina. SDF-1 blockade with a neutralizing antibody increased photoreceptor cell loss and macrophage accumulation in the subretinal space. The retinal precursor cell line R28 expressed CXCR4. SDF-1 rescued serum starvation-induced apoptosis in R28 cells and enhanced their ability to participate in wound closure in a scratch assay. Our results indicate a surprising, protective role for SDF-1 in RD. This effect may be mediated directly or indirectly through other cell types.
| Original language | English |
|---|---|
| Pages (from-to) | 2268-2277 |
| Number of pages | 10 |
| Journal | American Journal of Pathology |
| Volume | 177 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 2010 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
Cite this
Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment. / Otsuka, Hiroki; Arimura, Noboru; Sonoda, Shozo; Nakamura, Makoto; Hashiguchi, Teruto; Maruyama, Ikuro; nakao, shintaro; Hafezi-Moghadam, Ali; Sakamoto, Taiji.
In: American Journal of Pathology, Vol. 177, No. 5, 01.01.2010, p. 2268-2277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment
AU - Otsuka, Hiroki
AU - Arimura, Noboru
AU - Sonoda, Shozo
AU - Nakamura, Makoto
AU - Hashiguchi, Teruto
AU - Maruyama, Ikuro
AU - nakao, shintaro
AU - Hafezi-Moghadam, Ali
AU - Sakamoto, Taiji
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Stromal cell-derived factor-1 (SDF-1) causes chemotaxis of CXCR4-expressing bone marrow-derived cells. SDF-1 is involved in the pathogenesis of various vascular diseases, including those of the eye. However, the role of SDF-1 in neuronal diseases is not completely understood. Here, we show higher SDF-1 levels in the vitreous humor of patients with retinal detachment (RD) compared with normal patients. SDF-1 correlated positively with the duration as well as the extent of RD. Furthermore, SDF-1 correlated significantly with levels of interleukin-6 and interleukin-8, but not with vascular endothelial growth factor. Western blot analysis results showed significant SDF-1 up-regulation in detached rat retinas compared with normal animals. Immunohistochemistry data showed that SDF-1 was co-localized with the glial cells of the detached retina. SDF-1 blockade with a neutralizing antibody increased photoreceptor cell loss and macrophage accumulation in the subretinal space. The retinal precursor cell line R28 expressed CXCR4. SDF-1 rescued serum starvation-induced apoptosis in R28 cells and enhanced their ability to participate in wound closure in a scratch assay. Our results indicate a surprising, protective role for SDF-1 in RD. This effect may be mediated directly or indirectly through other cell types.
AB - Stromal cell-derived factor-1 (SDF-1) causes chemotaxis of CXCR4-expressing bone marrow-derived cells. SDF-1 is involved in the pathogenesis of various vascular diseases, including those of the eye. However, the role of SDF-1 in neuronal diseases is not completely understood. Here, we show higher SDF-1 levels in the vitreous humor of patients with retinal detachment (RD) compared with normal patients. SDF-1 correlated positively with the duration as well as the extent of RD. Furthermore, SDF-1 correlated significantly with levels of interleukin-6 and interleukin-8, but not with vascular endothelial growth factor. Western blot analysis results showed significant SDF-1 up-regulation in detached rat retinas compared with normal animals. Immunohistochemistry data showed that SDF-1 was co-localized with the glial cells of the detached retina. SDF-1 blockade with a neutralizing antibody increased photoreceptor cell loss and macrophage accumulation in the subretinal space. The retinal precursor cell line R28 expressed CXCR4. SDF-1 rescued serum starvation-induced apoptosis in R28 cells and enhanced their ability to participate in wound closure in a scratch assay. Our results indicate a surprising, protective role for SDF-1 in RD. This effect may be mediated directly or indirectly through other cell types.
UR - http://www.scopus.com/inward/record.url?scp=78149292859&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78149292859&partnerID=8YFLogxK
U2 - 10.2353/ajpath.2010.100134
DO - 10.2353/ajpath.2010.100134
M3 - Article
C2 - 20889568
AN - SCOPUS:78149292859
VL - 177
SP - 2268
EP - 2277
JO - American Journal of Pathology
JF - American Journal of Pathology
SN - 0002-9440
IS - 5
ER -