Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment

Hiroki Otsuka, Noboru Arimura, Shozo Sonoda, Makoto Nakamura, Teruto Hashiguchi, Ikuro Maruyama, shintaro nakao, Ali Hafezi-Moghadam, Taiji Sakamoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Stromal cell-derived factor-1 (SDF-1) causes chemotaxis of CXCR4-expressing bone marrow-derived cells. SDF-1 is involved in the pathogenesis of various vascular diseases, including those of the eye. However, the role of SDF-1 in neuronal diseases is not completely understood. Here, we show higher SDF-1 levels in the vitreous humor of patients with retinal detachment (RD) compared with normal patients. SDF-1 correlated positively with the duration as well as the extent of RD. Furthermore, SDF-1 correlated significantly with levels of interleukin-6 and interleukin-8, but not with vascular endothelial growth factor. Western blot analysis results showed significant SDF-1 up-regulation in detached rat retinas compared with normal animals. Immunohistochemistry data showed that SDF-1 was co-localized with the glial cells of the detached retina. SDF-1 blockade with a neutralizing antibody increased photoreceptor cell loss and macrophage accumulation in the subretinal space. The retinal precursor cell line R28 expressed CXCR4. SDF-1 rescued serum starvation-induced apoptosis in R28 cells and enhanced their ability to participate in wound closure in a scratch assay. Our results indicate a surprising, protective role for SDF-1 in RD. This effect may be mediated directly or indirectly through other cell types.

Original languageEnglish
Pages (from-to)2268-2277
Number of pages10
JournalAmerican Journal of Pathology
Volume177
Issue number5
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

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Chemokine CXCL12
Photoreceptor Cells
Cytoprotection
Retinal Detachment
Retina
Vitreous Body
Aptitude
Chemotaxis
Starvation
Neutralizing Antibodies
Interleukin-8
Vascular Diseases
Neuroglia
Bone Marrow Cells
Vascular Endothelial Growth Factor A
Interleukin-6
Up-Regulation
Western Blotting
Immunohistochemistry
Macrophages

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Otsuka, H., Arimura, N., Sonoda, S., Nakamura, M., Hashiguchi, T., Maruyama, I., ... Sakamoto, T. (2010). Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment. American Journal of Pathology, 177(5), 2268-2277. https://doi.org/10.2353/ajpath.2010.100134

Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment. / Otsuka, Hiroki; Arimura, Noboru; Sonoda, Shozo; Nakamura, Makoto; Hashiguchi, Teruto; Maruyama, Ikuro; nakao, shintaro; Hafezi-Moghadam, Ali; Sakamoto, Taiji.

In: American Journal of Pathology, Vol. 177, No. 5, 01.01.2010, p. 2268-2277.

Research output: Contribution to journalArticle

Otsuka, H, Arimura, N, Sonoda, S, Nakamura, M, Hashiguchi, T, Maruyama, I, nakao, S, Hafezi-Moghadam, A & Sakamoto, T 2010, 'Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment', American Journal of Pathology, vol. 177, no. 5, pp. 2268-2277. https://doi.org/10.2353/ajpath.2010.100134
Otsuka, Hiroki ; Arimura, Noboru ; Sonoda, Shozo ; Nakamura, Makoto ; Hashiguchi, Teruto ; Maruyama, Ikuro ; nakao, shintaro ; Hafezi-Moghadam, Ali ; Sakamoto, Taiji. / Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment. In: American Journal of Pathology. 2010 ; Vol. 177, No. 5. pp. 2268-2277.
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