Structural and biochemical bases of photorespiration in C4 plants: Quantification of organelles and glycine decarboxylase

Yasuyuki Yoshimura, Fumitake Kubota, Ueno Osamu

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

In C4 plants, photorespiration is decreased relative to C 3 plants. However, it remains unclear how much photorespiratory capacity C4 leaf tissues actually have. We thoroughly investigated the quantitative distribution of photorespiratory organelles and the immunogold localization of the P protein of glycine decarboxylase (GDC) in mesophyll (M) and bundle sheath (BS) cells of various C4 grass species. Specific differences occurred in the proportions of mitochondria and peroxisomes in the BS cells (relative to the M cells) in photosynthetic tissues surrounding a vein: lower in the NADP-malic enzyme (NADP-ME) species having poorly formed grana in the BS chloroplasts, and higher in the NAD-malic enzyme (NAD-ME) and phosphoenolpyruvate carboxykinase (PCK) species having well developed grana. In all C4 species, GDC was localized mainly in the BS mitochondria. When the total amounts of GDC in the BS mitochondria per unit leaf width were estimated from the immunogold labeling density and the quantity of mitochondria, the BSs of NADP-ME species contained less GDC than those of NAD-ME or PCK species. This trend was also verified by immunoblot analysis of leaf soluble protein. There was a high positive correlation between the degree of granal development (granal index) in the BS chloroplasts and the total amount of GDC in the BS mitochondria. The variations in the structural and biochemical features involved in photorespiration found among C4 species might reflect differences in the O2/CO2 partial pressure and in the potential photorespiratory capacity of the BS cells.

Original languageEnglish
Pages (from-to)307-317
Number of pages11
JournalPlanta
Volume220
Issue number2
DOIs
Publication statusPublished - Dec 1 2004

Fingerprint

Glycine Dehydrogenase (Decarboxylating)
photorespiration
C4 plants
Organelles
malic enzyme
organelles
malate dehydrogenase-(oxaloacetate-decarboxylating) (NAD+)
Mitochondria
mitochondria
bundle sheath cells
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
grana
Phosphoenolpyruvate
Thylakoids
Chloroplasts
chloroplasts
leaves
Peroxisomes
Partial Pressure
peroxisomes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Structural and biochemical bases of photorespiration in C4 plants : Quantification of organelles and glycine decarboxylase. / Yoshimura, Yasuyuki; Kubota, Fumitake; Osamu, Ueno.

In: Planta, Vol. 220, No. 2, 01.12.2004, p. 307-317.

Research output: Contribution to journalArticle

Yoshimura, Yasuyuki ; Kubota, Fumitake ; Osamu, Ueno. / Structural and biochemical bases of photorespiration in C4 plants : Quantification of organelles and glycine decarboxylase. In: Planta. 2004 ; Vol. 220, No. 2. pp. 307-317.
@article{7dcf306eefcc4961aefb7a3fac530210,
title = "Structural and biochemical bases of photorespiration in C4 plants: Quantification of organelles and glycine decarboxylase",
abstract = "In C4 plants, photorespiration is decreased relative to C 3 plants. However, it remains unclear how much photorespiratory capacity C4 leaf tissues actually have. We thoroughly investigated the quantitative distribution of photorespiratory organelles and the immunogold localization of the P protein of glycine decarboxylase (GDC) in mesophyll (M) and bundle sheath (BS) cells of various C4 grass species. Specific differences occurred in the proportions of mitochondria and peroxisomes in the BS cells (relative to the M cells) in photosynthetic tissues surrounding a vein: lower in the NADP-malic enzyme (NADP-ME) species having poorly formed grana in the BS chloroplasts, and higher in the NAD-malic enzyme (NAD-ME) and phosphoenolpyruvate carboxykinase (PCK) species having well developed grana. In all C4 species, GDC was localized mainly in the BS mitochondria. When the total amounts of GDC in the BS mitochondria per unit leaf width were estimated from the immunogold labeling density and the quantity of mitochondria, the BSs of NADP-ME species contained less GDC than those of NAD-ME or PCK species. This trend was also verified by immunoblot analysis of leaf soluble protein. There was a high positive correlation between the degree of granal development (granal index) in the BS chloroplasts and the total amount of GDC in the BS mitochondria. The variations in the structural and biochemical features involved in photorespiration found among C4 species might reflect differences in the O2/CO2 partial pressure and in the potential photorespiratory capacity of the BS cells.",
author = "Yasuyuki Yoshimura and Fumitake Kubota and Ueno Osamu",
year = "2004",
month = "12",
day = "1",
doi = "10.1007/s00425-004-1335-1",
language = "English",
volume = "220",
pages = "307--317",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Structural and biochemical bases of photorespiration in C4 plants

T2 - Quantification of organelles and glycine decarboxylase

AU - Yoshimura, Yasuyuki

AU - Kubota, Fumitake

AU - Osamu, Ueno

PY - 2004/12/1

Y1 - 2004/12/1

N2 - In C4 plants, photorespiration is decreased relative to C 3 plants. However, it remains unclear how much photorespiratory capacity C4 leaf tissues actually have. We thoroughly investigated the quantitative distribution of photorespiratory organelles and the immunogold localization of the P protein of glycine decarboxylase (GDC) in mesophyll (M) and bundle sheath (BS) cells of various C4 grass species. Specific differences occurred in the proportions of mitochondria and peroxisomes in the BS cells (relative to the M cells) in photosynthetic tissues surrounding a vein: lower in the NADP-malic enzyme (NADP-ME) species having poorly formed grana in the BS chloroplasts, and higher in the NAD-malic enzyme (NAD-ME) and phosphoenolpyruvate carboxykinase (PCK) species having well developed grana. In all C4 species, GDC was localized mainly in the BS mitochondria. When the total amounts of GDC in the BS mitochondria per unit leaf width were estimated from the immunogold labeling density and the quantity of mitochondria, the BSs of NADP-ME species contained less GDC than those of NAD-ME or PCK species. This trend was also verified by immunoblot analysis of leaf soluble protein. There was a high positive correlation between the degree of granal development (granal index) in the BS chloroplasts and the total amount of GDC in the BS mitochondria. The variations in the structural and biochemical features involved in photorespiration found among C4 species might reflect differences in the O2/CO2 partial pressure and in the potential photorespiratory capacity of the BS cells.

AB - In C4 plants, photorespiration is decreased relative to C 3 plants. However, it remains unclear how much photorespiratory capacity C4 leaf tissues actually have. We thoroughly investigated the quantitative distribution of photorespiratory organelles and the immunogold localization of the P protein of glycine decarboxylase (GDC) in mesophyll (M) and bundle sheath (BS) cells of various C4 grass species. Specific differences occurred in the proportions of mitochondria and peroxisomes in the BS cells (relative to the M cells) in photosynthetic tissues surrounding a vein: lower in the NADP-malic enzyme (NADP-ME) species having poorly formed grana in the BS chloroplasts, and higher in the NAD-malic enzyme (NAD-ME) and phosphoenolpyruvate carboxykinase (PCK) species having well developed grana. In all C4 species, GDC was localized mainly in the BS mitochondria. When the total amounts of GDC in the BS mitochondria per unit leaf width were estimated from the immunogold labeling density and the quantity of mitochondria, the BSs of NADP-ME species contained less GDC than those of NAD-ME or PCK species. This trend was also verified by immunoblot analysis of leaf soluble protein. There was a high positive correlation between the degree of granal development (granal index) in the BS chloroplasts and the total amount of GDC in the BS mitochondria. The variations in the structural and biochemical features involved in photorespiration found among C4 species might reflect differences in the O2/CO2 partial pressure and in the potential photorespiratory capacity of the BS cells.

UR - http://www.scopus.com/inward/record.url?scp=11844293501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11844293501&partnerID=8YFLogxK

U2 - 10.1007/s00425-004-1335-1

DO - 10.1007/s00425-004-1335-1

M3 - Article

C2 - 15290293

AN - SCOPUS:11844293501

VL - 220

SP - 307

EP - 317

JO - Planta

JF - Planta

SN - 0032-0935

IS - 2

ER -