Structural and biochemical characterization of the C 3-C 4 intermediate Brassica gravinae and relatives, with particular reference to cellular distribution of Rubisco

Ueno Osamu

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Abstract

On the basis of its CO 2 compensation concentration, Brassica gravinae Ten. has been reported to be a C 3-C 4 intermediate. This study investigated the structural and biochemical features of photosynthetic metabolism in B. gravinae. The cellular distribution of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) was also examined in B. gravinae, B. napus L. (C 3), Raphanus sativus L. (C 3), and Diplotaxis tenuifolia (L.) DC. (C 3-C 4) by immunogold electron microscopy to elucidate Rubisco expression during the evolution from C 3 to C 3-C 4 intermediate plants. The bundle sheath (BS) cells of B. gravinae contained centrifugally located chloroplasts as well as centripetally located chloroplasts and mitochondria. Glycine decarboxylase P-protein was localized in the BS mitochondria. Brassica gravinae had low C 4 enzyme activities and high activities of Rubisco and photorespiratory enzymes, suggesting that it reduces photorespiratory CO 2 loss by the glycine shuttle. In B. gravinae, the labelling density of Rubisco was higher in the mesophyll chloroplasts than in the BS chloroplasts. A similar cellular pattern was found in other Brassicaceae species. These data demonstrate that, during the evolution from C 3 to C 3-C 4 intermediate plants, the intercellular pattern of Rubisco expression did not change greatly, although the amount of chloroplasts in the BS cells increased. It also appears that intracellular variation in Rubisco distribution may occur within the BS cells of B. gravinae.

Original languageEnglish
Pages (from-to)5347-5355
Number of pages9
JournalJournal of Experimental Botany
Volume62
Issue number15
DOIs
Publication statusPublished - Nov 1 2011

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Brassica gravinae
Oxygenases
Brassica
ribulose 1,5-diphosphate
oxygenases
Chloroplasts
chloroplasts
bundle sheath cells
Carbon Monoxide
Glycine Dehydrogenase (Decarboxylating)
Mitochondria
mitochondria
Brassicaceae
Diplotaxis tenuifolia
Raphanus
Enzymes
Raphanus sativus
Glycine
ribulose-1,5 diphosphate
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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abstract = "On the basis of its CO 2 compensation concentration, Brassica gravinae Ten. has been reported to be a C 3-C 4 intermediate. This study investigated the structural and biochemical features of photosynthetic metabolism in B. gravinae. The cellular distribution of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) was also examined in B. gravinae, B. napus L. (C 3), Raphanus sativus L. (C 3), and Diplotaxis tenuifolia (L.) DC. (C 3-C 4) by immunogold electron microscopy to elucidate Rubisco expression during the evolution from C 3 to C 3-C 4 intermediate plants. The bundle sheath (BS) cells of B. gravinae contained centrifugally located chloroplasts as well as centripetally located chloroplasts and mitochondria. Glycine decarboxylase P-protein was localized in the BS mitochondria. Brassica gravinae had low C 4 enzyme activities and high activities of Rubisco and photorespiratory enzymes, suggesting that it reduces photorespiratory CO 2 loss by the glycine shuttle. In B. gravinae, the labelling density of Rubisco was higher in the mesophyll chloroplasts than in the BS chloroplasts. A similar cellular pattern was found in other Brassicaceae species. These data demonstrate that, during the evolution from C 3 to C 3-C 4 intermediate plants, the intercellular pattern of Rubisco expression did not change greatly, although the amount of chloroplasts in the BS cells increased. It also appears that intracellular variation in Rubisco distribution may occur within the BS cells of B. gravinae.",
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T1 - Structural and biochemical characterization of the C 3-C 4 intermediate Brassica gravinae and relatives, with particular reference to cellular distribution of Rubisco

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N2 - On the basis of its CO 2 compensation concentration, Brassica gravinae Ten. has been reported to be a C 3-C 4 intermediate. This study investigated the structural and biochemical features of photosynthetic metabolism in B. gravinae. The cellular distribution of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) was also examined in B. gravinae, B. napus L. (C 3), Raphanus sativus L. (C 3), and Diplotaxis tenuifolia (L.) DC. (C 3-C 4) by immunogold electron microscopy to elucidate Rubisco expression during the evolution from C 3 to C 3-C 4 intermediate plants. The bundle sheath (BS) cells of B. gravinae contained centrifugally located chloroplasts as well as centripetally located chloroplasts and mitochondria. Glycine decarboxylase P-protein was localized in the BS mitochondria. Brassica gravinae had low C 4 enzyme activities and high activities of Rubisco and photorespiratory enzymes, suggesting that it reduces photorespiratory CO 2 loss by the glycine shuttle. In B. gravinae, the labelling density of Rubisco was higher in the mesophyll chloroplasts than in the BS chloroplasts. A similar cellular pattern was found in other Brassicaceae species. These data demonstrate that, during the evolution from C 3 to C 3-C 4 intermediate plants, the intercellular pattern of Rubisco expression did not change greatly, although the amount of chloroplasts in the BS cells increased. It also appears that intracellular variation in Rubisco distribution may occur within the BS cells of B. gravinae.

AB - On the basis of its CO 2 compensation concentration, Brassica gravinae Ten. has been reported to be a C 3-C 4 intermediate. This study investigated the structural and biochemical features of photosynthetic metabolism in B. gravinae. The cellular distribution of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) was also examined in B. gravinae, B. napus L. (C 3), Raphanus sativus L. (C 3), and Diplotaxis tenuifolia (L.) DC. (C 3-C 4) by immunogold electron microscopy to elucidate Rubisco expression during the evolution from C 3 to C 3-C 4 intermediate plants. The bundle sheath (BS) cells of B. gravinae contained centrifugally located chloroplasts as well as centripetally located chloroplasts and mitochondria. Glycine decarboxylase P-protein was localized in the BS mitochondria. Brassica gravinae had low C 4 enzyme activities and high activities of Rubisco and photorespiratory enzymes, suggesting that it reduces photorespiratory CO 2 loss by the glycine shuttle. In B. gravinae, the labelling density of Rubisco was higher in the mesophyll chloroplasts than in the BS chloroplasts. A similar cellular pattern was found in other Brassicaceae species. These data demonstrate that, during the evolution from C 3 to C 3-C 4 intermediate plants, the intercellular pattern of Rubisco expression did not change greatly, although the amount of chloroplasts in the BS cells increased. It also appears that intracellular variation in Rubisco distribution may occur within the BS cells of B. gravinae.

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