TY - JOUR
T1 - Structural and morphological transition of long-chain phospholipid vesicles induced by mixing with short-chain phospholipid
AU - Takajo, Yuichi
AU - Matsuki, Hitoshi
AU - Matsubara, Hiroki
AU - Tsuchiya, Koji
AU - Aratono, Makoto
AU - Yamanaka, Michio
N1 - Funding Information:
This work was supported in part by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (No. 20 5174). The authors wish to express their gratitude to Prof. Masahiko Abe and Prof. Hideki Sakai of Tokyo University of Science for permission to perform the cryo-TEM experiments at the facility of their research group.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4/1
Y1 - 2010/4/1
N2 - Effects of a short-chain phospholipid, dihexanoylphosphatidylcholine (DHPC), on the structure and morphology of membrane assemblies of a long-chain phospholipid, dimyristoylphosphatidylcholine (DMPC), were examined by fluorescence spectroscopy, differential scanning calorimetry (DSC), and cryogenic transmission electron microscopy (cryo-TEM). It was found by fluorescence measurements that DHPC affects on the gel and liquid crystalline state of DMPC vesicle membranes in different ways. Further, the result of DSC suggested that, along the transition process from DMPC vesicle to DMPC-DHPC mixed micelle, there are at least three different concentration regions which are characterized by the individual variation pattern of the transition temperature and enthalpy change. The cryo-TEM micrographs demonstrated the formation of thread-like assemblies in the second region and the coexistence of the assemblies and spherical micelles in the third region. Thus, it was concluded that the structural transition from DMPC vesicle to DMPC-DHPC mixed micelle could occur in a stepwise manner through the formation of the thread-like assembly, which cannot be described by the three-stage model of vesicle to micelle transition.
AB - Effects of a short-chain phospholipid, dihexanoylphosphatidylcholine (DHPC), on the structure and morphology of membrane assemblies of a long-chain phospholipid, dimyristoylphosphatidylcholine (DMPC), were examined by fluorescence spectroscopy, differential scanning calorimetry (DSC), and cryogenic transmission electron microscopy (cryo-TEM). It was found by fluorescence measurements that DHPC affects on the gel and liquid crystalline state of DMPC vesicle membranes in different ways. Further, the result of DSC suggested that, along the transition process from DMPC vesicle to DMPC-DHPC mixed micelle, there are at least three different concentration regions which are characterized by the individual variation pattern of the transition temperature and enthalpy change. The cryo-TEM micrographs demonstrated the formation of thread-like assemblies in the second region and the coexistence of the assemblies and spherical micelles in the third region. Thus, it was concluded that the structural transition from DMPC vesicle to DMPC-DHPC mixed micelle could occur in a stepwise manner through the formation of the thread-like assembly, which cannot be described by the three-stage model of vesicle to micelle transition.
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U2 - 10.1016/j.colsurfb.2009.12.025
DO - 10.1016/j.colsurfb.2009.12.025
M3 - Article
C2 - 20097547
AN - SCOPUS:75749151697
SN - 0927-7765
VL - 76
SP - 571
EP - 576
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 2
ER -