Structural and morphological transition of long-chain phospholipid vesicles induced by mixing with short-chain phospholipid

Yuichi Takajo, Hitoshi Matsuki, Hiroki Matsubara, Koji Tsuchiya, Makoto Aratono, Michio Yamanaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Effects of a short-chain phospholipid, dihexanoylphosphatidylcholine (DHPC), on the structure and morphology of membrane assemblies of a long-chain phospholipid, dimyristoylphosphatidylcholine (DMPC), were examined by fluorescence spectroscopy, differential scanning calorimetry (DSC), and cryogenic transmission electron microscopy (cryo-TEM). It was found by fluorescence measurements that DHPC affects on the gel and liquid crystalline state of DMPC vesicle membranes in different ways. Further, the result of DSC suggested that, along the transition process from DMPC vesicle to DMPC-DHPC mixed micelle, there are at least three different concentration regions which are characterized by the individual variation pattern of the transition temperature and enthalpy change. The cryo-TEM micrographs demonstrated the formation of thread-like assemblies in the second region and the coexistence of the assemblies and spherical micelles in the third region. Thus, it was concluded that the structural transition from DMPC vesicle to DMPC-DHPC mixed micelle could occur in a stepwise manner through the formation of the thread-like assembly, which cannot be described by the three-stage model of vesicle to micelle transition.

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume76
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

Fingerprint

Dimyristoylphosphatidylcholine
Phospholipids
Micelles
micelles
assemblies
threads
Cryogenics
cryogenics
Differential scanning calorimetry
heat measurement
Differential Scanning Calorimetry
membranes
Transmission electron microscopy
Membranes
Transmission Electron Microscopy
fluorescence
transmission electron microscopy
scanning
Fluorescence spectroscopy
Superconducting transition temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Structural and morphological transition of long-chain phospholipid vesicles induced by mixing with short-chain phospholipid. / Takajo, Yuichi; Matsuki, Hitoshi; Matsubara, Hiroki; Tsuchiya, Koji; Aratono, Makoto; Yamanaka, Michio.

In: Colloids and Surfaces B: Biointerfaces, Vol. 76, No. 2, 01.04.2010, p. 571-576.

Research output: Contribution to journalArticle

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AU - Yamanaka, Michio

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