Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor

Mapping the odorant-binding site

Sayako Katada, Takatsugu Hirokawa, Yuki Oka, Makiko Suwa, Kazushige Touhara

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

The olfactory receptor (OR) superfamily provides a basis for the remarkable ability to recognize and discriminate a large number of odorants. In mice, the superfamily includes ∼1000 members, and they recognize overlapping sets of odorants with distinct affinities and specificities. To address the molecular basis of odor discrimination by the mammalian OR superfamily, we performed functional analysis on a series of site-directed mutants and performed ligand docking simulation studies to define the odorant-binding site of a mouse OR. Our results indicate that several amino acids in the transmembrane domains formed a ligand-binding pocket. Although other G-protein-coupled receptors (GPCRs) recognize biogenic ligands mainly with ionic or hydrogen bonding interactions, ORs recognize odorants mostly via hydrophobic and van der Waals interactions. This accounts for the broad but selective binding by ORs as well as their relatively low ligand-binding affinities. Furthermore, we succeeded in rational receptor design, inserting point mutations in the odorant-binding site that resulted in predicted changes in ligand specificity and antagonist activity. This ability to rationally design the receptor validated the binding site structure that was deduced with our mutational and ligand docking studies. Such broad and specific sensitivity suggests an evolutionary process during which mutations in the active site led to an enormous number of ORs with a wide range of ligand specificity. The current study reveals the molecular environment of the odorant-binding site, and it further advances the understanding of GPCR pharmacology.

Original languageEnglish
Pages (from-to)1806-1815
Number of pages10
JournalJournal of Neuroscience
Volume25
Issue number7
DOIs
Publication statusPublished - Feb 16 2005

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Odorant Receptors
Binding Sites
Ligands
G-Protein-Coupled Receptors
Hydrogen Bonding
Odorants
Point Mutation
Catalytic Domain
Pharmacology
Amino Acids
Mutation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor : Mapping the odorant-binding site. / Katada, Sayako; Hirokawa, Takatsugu; Oka, Yuki; Suwa, Makiko; Touhara, Kazushige.

In: Journal of Neuroscience, Vol. 25, No. 7, 16.02.2005, p. 1806-1815.

Research output: Contribution to journalArticle

Katada, Sayako ; Hirokawa, Takatsugu ; Oka, Yuki ; Suwa, Makiko ; Touhara, Kazushige. / Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor : Mapping the odorant-binding site. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 7. pp. 1806-1815.
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