Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region

Takahiro Yoshinaka, Hidetaka Kosako, Takuma Yoshizumi, Ryo Furukawa, Yu Hirano, Osamu Kuge, Taro Tamada, Takumi Koshiba

Research output: Contribution to journalArticle

Abstract

The coiled-coil motif mediates subunit oligomerization and scaffolding and underlies several fundamental biologic processes. Prohibitins (PHBs), mitochondrial inner membrane proteins involved in mitochondrial homeostasis and signal transduction, are predicted to have a coiled-coil motif, but their structural features are poorly understood. Here we solved the crystal structure of the heptad repeat (HR) region of PHB2 at 1.7-Å resolution, showing that it assembles into a dimeric, antiparallel coiled-coil with a unique negatively charged area essential for the PHB interactome in mitochondria. Disruption of the HR coiled-coil abolishes well-ordered PHB complexes and the mitochondrial tubular networks accompanying PHB-dependent signaling. Using a proximity-dependent biotin identification (BioID) technique in live cells, we mapped a number of mitochondrial intermembrane space proteins whose association with PHB2 relies on the HR coiled-coil region. Elucidation of the PHB complex structure in mitochondria provides insight into essential PHB interactomes required for mitochondrial dynamics as well as signal transduction.

Original languageEnglish
Pages (from-to)1065-1078
Number of pages14
JournaliScience
Volume19
DOIs
Publication statusPublished - Sep 27 2019

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Signal Transduction
Mitochondria
Mitochondrial Dynamics
Biotin
prohibitin
Membrane Proteins
Homeostasis
Proteins

All Science Journal Classification (ASJC) codes

  • General

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Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes : Insight into a Role of the Coiled-Coil Region. / Yoshinaka, Takahiro; Kosako, Hidetaka; Yoshizumi, Takuma; Furukawa, Ryo; Hirano, Yu; Kuge, Osamu; Tamada, Taro; Koshiba, Takumi.

In: iScience, Vol. 19, 27.09.2019, p. 1065-1078.

Research output: Contribution to journalArticle

Yoshinaka, T, Kosako, H, Yoshizumi, T, Furukawa, R, Hirano, Y, Kuge, O, Tamada, T & Koshiba, T 2019, 'Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region', iScience, vol. 19, pp. 1065-1078. https://doi.org/10.1016/j.isci.2019.08.056
Yoshinaka, Takahiro ; Kosako, Hidetaka ; Yoshizumi, Takuma ; Furukawa, Ryo ; Hirano, Yu ; Kuge, Osamu ; Tamada, Taro ; Koshiba, Takumi. / Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes : Insight into a Role of the Coiled-Coil Region. In: iScience. 2019 ; Vol. 19. pp. 1065-1078.
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