Structural basis of mitochondrial tethering by mitofusin complexes

Takumi Koshiba, Scott A. Detmer, Jens T. Kaiser, Hsiuchen Chen, J. Michael McCaffery, David C. Chan

Research output: Contribution to journalArticle

514 Citations (Scopus)

Abstract

Vesicle fusion involves vesicle tethering, docking, and membrane merger. We show that mitofusin, an integral mitochondrial membrane protein, is required on adjacent mitochondria to mediate fusion, which indicates that mitofusin complexes act in trans (that is, between adjacent mitochondria). A heptad repeat region (HR2) mediates mitofusin oligomerization by assembling a dimeric, antiparallel coiled coil. The transmembrane segments are located at opposite ends of the 95 angstrom coiled coil and provide a mechanism for organelle tethering. Consistent with this proposal, truncated mitofusin, in an HR2-dependent manner, causes mitochondria to become apposed with a uniform gap. Our results suggest that HR2 functions as a mitochondrial tether before fusion.

Original languageEnglish
Pages (from-to)858-862
Number of pages5
JournalScience
Volume305
Issue number5685
DOIs
Publication statusPublished - Aug 6 2004

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Mitochondria
Mitochondrial Proteins
Mitochondrial Membranes
Organelles
Membrane Proteins
Membranes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Koshiba, T., Detmer, S. A., Kaiser, J. T., Chen, H., McCaffery, J. M., & Chan, D. C. (2004). Structural basis of mitochondrial tethering by mitofusin complexes. Science, 305(5685), 858-862. https://doi.org/10.1126/science.1099793

Structural basis of mitochondrial tethering by mitofusin complexes. / Koshiba, Takumi; Detmer, Scott A.; Kaiser, Jens T.; Chen, Hsiuchen; McCaffery, J. Michael; Chan, David C.

In: Science, Vol. 305, No. 5685, 06.08.2004, p. 858-862.

Research output: Contribution to journalArticle

Koshiba, T, Detmer, SA, Kaiser, JT, Chen, H, McCaffery, JM & Chan, DC 2004, 'Structural basis of mitochondrial tethering by mitofusin complexes', Science, vol. 305, no. 5685, pp. 858-862. https://doi.org/10.1126/science.1099793
Koshiba T, Detmer SA, Kaiser JT, Chen H, McCaffery JM, Chan DC. Structural basis of mitochondrial tethering by mitofusin complexes. Science. 2004 Aug 6;305(5685):858-862. https://doi.org/10.1126/science.1099793
Koshiba, Takumi ; Detmer, Scott A. ; Kaiser, Jens T. ; Chen, Hsiuchen ; McCaffery, J. Michael ; Chan, David C. / Structural basis of mitochondrial tethering by mitofusin complexes. In: Science. 2004 ; Vol. 305, No. 5685. pp. 858-862.
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