Structural basis of replication origin recognition by the DnaA protein

Norie Fujikawa, Hitoshi Kurumizaka, Osamu Nureki, Takaho Terada, Mikako Shirouzu, Tsutomu Katayama, Shigeyuki Yokoyama

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Escherichia coli DnaA binds to 9 bp sequences (DnaA boxes) in the replication origin, oriC, to form a complex initiating chromosomal DNA replication. In the present study, we determined the crystal structure of its DNA-binding domain (domain IV) complexed with a DnaA box at 2.1 Å resolution. DnaA domain IV contains a helix-turn-helix motif for DNA binding. One helix and a loop of the helix-turn-helix motif are inserted into the major groove and 5 bp (3′ two-thirds of the DnaA box sequence) are recognized through base-specific hydrogen bonds and van der Waals contacts with the C5-methyl groups of thymines. In the minor groove, Arg399, located in the loop adjacent to the motif, recognizes three more base pairs (5′ one-third of the DnaA box sequence) by base-specific hydrogen bonds. DNA bending by ∼28° was also observed in the complex. These base-specific interactions explain how DnaA exhibits higher affinity for the strong DnaA boxes (R1, R2 and R4) than the weak DnaA boxes (R3 and M) in the replication origin.

Original languageEnglish
Pages (from-to)2077-2086
Number of pages10
JournalNucleic acids research
Volume31
Issue number8
DOIs
Publication statusPublished - Apr 15 2003

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Replication Origin
Helix-Turn-Helix Motifs
Hydrogen
DNA
Proteins
Thymine
DNA Replication
Base Pairing
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Fujikawa, N., Kurumizaka, H., Nureki, O., Terada, T., Shirouzu, M., Katayama, T., & Yokoyama, S. (2003). Structural basis of replication origin recognition by the DnaA protein. Nucleic acids research, 31(8), 2077-2086. https://doi.org/10.1093/nar/gkg309

Structural basis of replication origin recognition by the DnaA protein. / Fujikawa, Norie; Kurumizaka, Hitoshi; Nureki, Osamu; Terada, Takaho; Shirouzu, Mikako; Katayama, Tsutomu; Yokoyama, Shigeyuki.

In: Nucleic acids research, Vol. 31, No. 8, 15.04.2003, p. 2077-2086.

Research output: Contribution to journalArticle

Fujikawa, N, Kurumizaka, H, Nureki, O, Terada, T, Shirouzu, M, Katayama, T & Yokoyama, S 2003, 'Structural basis of replication origin recognition by the DnaA protein', Nucleic acids research, vol. 31, no. 8, pp. 2077-2086. https://doi.org/10.1093/nar/gkg309
Fujikawa N, Kurumizaka H, Nureki O, Terada T, Shirouzu M, Katayama T et al. Structural basis of replication origin recognition by the DnaA protein. Nucleic acids research. 2003 Apr 15;31(8):2077-2086. https://doi.org/10.1093/nar/gkg309
Fujikawa, Norie ; Kurumizaka, Hitoshi ; Nureki, Osamu ; Terada, Takaho ; Shirouzu, Mikako ; Katayama, Tsutomu ; Yokoyama, Shigeyuki. / Structural basis of replication origin recognition by the DnaA protein. In: Nucleic acids research. 2003 ; Vol. 31, No. 8. pp. 2077-2086.
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