Structural basis of the 3′-end recognition of a leading strand in stalled replication forks by PriA

Kaori Tabata, Toyoyuki Ose, Naoaki Okamoto, Katsumi Maenaka, Taku Tanaka, Hisao Masai, Mihoko Saito, Tsuyoshi Shirai, Daisuke Kohda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In eubacteria, PriA helicase detects the stalled DNA replication forks. This critical role of PriA is ascribed to its ability to bind to the 3′ end of a nascent leading DNA strand in the stalled replication forks. The crystal structures in complexes with oligonucleotides and the combination of fluorescence correlation spectroscopy and mutagenesis reveal that the N-terminal domain of PriA possesses a binding pocket for the 3′-terminal nucleotide residue of DNA. The interaction with the deoxyribose 3′-OH is essential for the 3′-terminal recognition. In contrast, the direct interaction with 3′-end nucleobase is unexpected, considering the same affinity for oligonucleotides carrying the four bases at the 3′ end. Thus, the N-terminal domain of PriA recognizes the 3′-end base in a base-non-selective manner, in addition to the deoxyribose and 5′-side phosphodiester group, of the 3′-terminal nucleotide to acquire both sufficient affinity and non-selectivity to find all of the stalled replication forks generated during DNA duplication. This unique feature is prerequisite for the proper positioning of the helicase domain of PriA on the unreplicated double-stranded DNA.

Original languageEnglish
Pages (from-to)2584-2593
Number of pages10
JournalEMBO Journal
Volume26
Issue number10
DOIs
Publication statusPublished - May 16 2007

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DNA
Oligonucleotides
Nucleotides
Deoxyribose
Fluorescence Spectrometry
DNA Replication
Mutagenesis
Bacteria
Crystal structure
Fluorescence
Spectroscopy
3-deoxyribose

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Structural basis of the 3′-end recognition of a leading strand in stalled replication forks by PriA. / Tabata, Kaori; Ose, Toyoyuki; Okamoto, Naoaki; Maenaka, Katsumi; Tanaka, Taku; Masai, Hisao; Saito, Mihoko; Shirai, Tsuyoshi; Kohda, Daisuke.

In: EMBO Journal, Vol. 26, No. 10, 16.05.2007, p. 2584-2593.

Research output: Contribution to journalArticle

Tabata, K, Ose, T, Okamoto, N, Maenaka, K, Tanaka, T, Masai, H, Saito, M, Shirai, T & Kohda, D 2007, 'Structural basis of the 3′-end recognition of a leading strand in stalled replication forks by PriA', EMBO Journal, vol. 26, no. 10, pp. 2584-2593. https://doi.org/10.1038/sj.emboj.7601697
Tabata, Kaori ; Ose, Toyoyuki ; Okamoto, Naoaki ; Maenaka, Katsumi ; Tanaka, Taku ; Masai, Hisao ; Saito, Mihoko ; Shirai, Tsuyoshi ; Kohda, Daisuke. / Structural basis of the 3′-end recognition of a leading strand in stalled replication forks by PriA. In: EMBO Journal. 2007 ; Vol. 26, No. 10. pp. 2584-2593.
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