Structural basis of tRNA agmatinylation essential for AUA codon decoding

Takuo Osawa, Satoshi Kimura, Naohiro Terasaka, Hideko Inanaga, Tsutomu Suzuki, Tomoyuki Numata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The cytidine at the first position of the anticodon (C34) in the AUA codon-specific archaeal tRNA Ile2 is modified to 2-agmatinylcytidine (agm 2 C or agmatidine), an agmatine-conjugated cytidine derivative, which is crucial for the precise decoding of the genetic code. Agm 2 C is synthesized by tRNA Ile -agm 2 C synthetase (TiaS) in an ATP-dependent manner. Here we present the crystal structures of the Archaeoglobus fulgidus TiaS-tRNA Ile 2 complexed with ATP, or with AMPCPP and agmatine, revealing a previously unknown kinase module required for activating C34 by phosphorylation, and showing the molecular mechanism by which TiaS discriminates between tRNA Ile 2 and tRNA Met. In the TiaS-tRNA Ile 2-ATP complex, C34 is trapped within a pocket far away from the ATP-binding site. In the agmatine-containing crystals, C34 is located near the AMPCPP γ-phosphate in the kinase module, demonstrating that agmatine is essential for placing C34 in the active site. These observations also provide the structural dynamics for agm 2 C formation.

Original languageEnglish
Pages (from-to)1275-1280
Number of pages6
JournalNature Structural and Molecular Biology
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 1 2011
Externally publishedYes

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RNA, Transfer, Ile
Agmatine
Transfer RNA
Codon
Adenosine Triphosphate
Cytidine
Archaeoglobus fulgidus
Phosphotransferases
Genetic Code
Anticodon
Ligases
Catalytic Domain
Phosphates
Binding Sites
Phosphorylation
agmatidine

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Structural basis of tRNA agmatinylation essential for AUA codon decoding. / Osawa, Takuo; Kimura, Satoshi; Terasaka, Naohiro; Inanaga, Hideko; Suzuki, Tsutomu; Numata, Tomoyuki.

In: Nature Structural and Molecular Biology, Vol. 18, No. 11, 01.11.2011, p. 1275-1280.

Research output: Contribution to journalArticle

Osawa, Takuo ; Kimura, Satoshi ; Terasaka, Naohiro ; Inanaga, Hideko ; Suzuki, Tsutomu ; Numata, Tomoyuki. / Structural basis of tRNA agmatinylation essential for AUA codon decoding. In: Nature Structural and Molecular Biology. 2011 ; Vol. 18, No. 11. pp. 1275-1280.
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