Structural characterization of an aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori

Kohji Yamamoto, Akifumi Higashiura, Mamoru Suzuki, Takahiro Shiotsuki, Ryohei Sugahara, Takeshi Fujii, Atsushi Nakagawa

Research output: Contribution to journalArticle

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Abstract

We report a new member of the aldo-keto reductase (AKR) superfamily in the silkworm Bombyx mori. Based on its amino acid sequence, the new enzyme belongs to the AKR2 family and was previously assigned the systematic name AKR2E5. In the present study, recombinant AKR2E5 was expressed, purified to homogeneity, and characterized. The X-ray crystal structures were determined at 2.2 Å for the apoenzyme and at 2.3 Å resolution for the NADPH-AKR2E5 complex. Our results demonstrate that AKR2E5 is a 40-kDa monomer and includes the TIM- or (β/α)8-barrel typical for other AKRs. We found that AKR2E5 uses NADPH as a cosubstrate to reduce carbonyl compounds such as DL-glyceraldehyde, xylose, 3-hydroxy benzaldehyde, 17α-hydroxy progesterone, 11-hexadecenal, and bombykal. No NADH-dependent activity was detected. Site-directed mutagenesis of AKR2E5 indicates that amino acid residues Asp70, Tyr75, Lys104, and His137 contribute to catalytic activity, which is consistent with the data on other AKRs. To the best of our knowledge, AKR2E5 is only the second AKR characterized in silkworm. Our data should contribute to further understanding of the functional activity of insect AKRs.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume474
Issue number1
DOIs
Publication statusPublished - May 20 2016

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Bombyx
NADP
Glyceraldehyde
Apoenzymes
Amino Acids
Carbonyl compounds
Mutagenesis
Xylose
NAD
Progesterone
Catalyst activity
Monomers
Crystal structure
Site-Directed Mutagenesis
X rays
Names
Insects
Amino Acid Sequence
Enzymes
X-Rays

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Structural characterization of an aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori. / Yamamoto, Kohji; Higashiura, Akifumi; Suzuki, Mamoru; Shiotsuki, Takahiro; Sugahara, Ryohei; Fujii, Takeshi; Nakagawa, Atsushi.

In: Biochemical and Biophysical Research Communications, Vol. 474, No. 1, 20.05.2016, p. 104-110.

Research output: Contribution to journalArticle

Yamamoto, Kohji ; Higashiura, Akifumi ; Suzuki, Mamoru ; Shiotsuki, Takahiro ; Sugahara, Ryohei ; Fujii, Takeshi ; Nakagawa, Atsushi. / Structural characterization of an aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 474, No. 1. pp. 104-110.
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