Structural characterization of porcine enteropeptidase

Masashi Matsushima, Masao Ichinose, Naohisa Yahagi, Nobuyuki Kakei, Shinko Tsukada, Kazumasa Miki, Kiyoshi Kurokawa, Kosuke Tashiro, Koichiro Shiokawa, Kazuko Shinomiya, Hideaki Umeyama, Hideshi Inoue, Takayuki Takahashi, Kenji Takahashi

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Enteropeptidase (EC 3.4.21.9) is a key enzyme in the intestinal digestion cascade responsible for the conversion of trypsinogen to trypsin, which then activates various pancreatic zymogens. In order to structurally characterize the enzyme, we purified the enzyme from porcine duodenal mucosa and showed that it consists of three polypeptide chains, which we named 'mini' chain (M chain), light chain (L chain), and heavy chain (H chain) in order of increasing molecular size. Based on their NH2-terminal sequences, a cDNA clone for porcine enteropeptidase was isolated and analyzed. The clone was 3597 base pairs long, which encoded 1034 amino acid residues of a single- chain precursor form of enteropeptidase. The precursor contained an additional NH2-terminal 51-residue sequence including a putative internal signal sequence, followed by the M chain (66 residues), the H chain (682 residues), and the L chain (235 residues) in that order. The H chain had regions partially homologous in sequence with low density lipoprotein receptor and complement components. On the other hand, the L chain was highly homologous with the catalytic domains of trypsin-like serine proteinases. The structural model of the L chain suggests that the sequence, Arg885-Arg- Arg-Lys888, is probably involved in the unique substrate specificity of the enzyme, preferring acidic amino acid residues at the P2-P5 sites.

Original languageEnglish
Pages (from-to)19976-19982
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number31
Publication statusPublished - Aug 5 1994
Externally publishedYes

Fingerprint

Enteropeptidase
Swine
Light
Enzymes
Trypsin
arginylarginine
Clone Cells
Trypsinogen
Acidic Amino Acids
Enzyme Precursors
LDL Receptors
Structural Models
Serine Proteases
Sequence Homology
Protein Sorting Signals
Substrate Specificity
Base Pairing
Digestion
Catalytic Domain
Mucous Membrane

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Matsushima, M., Ichinose, M., Yahagi, N., Kakei, N., Tsukada, S., Miki, K., ... Takahashi, K. (1994). Structural characterization of porcine enteropeptidase. Journal of Biological Chemistry, 269(31), 19976-19982.

Structural characterization of porcine enteropeptidase. / Matsushima, Masashi; Ichinose, Masao; Yahagi, Naohisa; Kakei, Nobuyuki; Tsukada, Shinko; Miki, Kazumasa; Kurokawa, Kiyoshi; Tashiro, Kosuke; Shiokawa, Koichiro; Shinomiya, Kazuko; Umeyama, Hideaki; Inoue, Hideshi; Takahashi, Takayuki; Takahashi, Kenji.

In: Journal of Biological Chemistry, Vol. 269, No. 31, 05.08.1994, p. 19976-19982.

Research output: Contribution to journalArticle

Matsushima, M, Ichinose, M, Yahagi, N, Kakei, N, Tsukada, S, Miki, K, Kurokawa, K, Tashiro, K, Shiokawa, K, Shinomiya, K, Umeyama, H, Inoue, H, Takahashi, T & Takahashi, K 1994, 'Structural characterization of porcine enteropeptidase', Journal of Biological Chemistry, vol. 269, no. 31, pp. 19976-19982.
Matsushima M, Ichinose M, Yahagi N, Kakei N, Tsukada S, Miki K et al. Structural characterization of porcine enteropeptidase. Journal of Biological Chemistry. 1994 Aug 5;269(31):19976-19982.
Matsushima, Masashi ; Ichinose, Masao ; Yahagi, Naohisa ; Kakei, Nobuyuki ; Tsukada, Shinko ; Miki, Kazumasa ; Kurokawa, Kiyoshi ; Tashiro, Kosuke ; Shiokawa, Koichiro ; Shinomiya, Kazuko ; Umeyama, Hideaki ; Inoue, Hideshi ; Takahashi, Takayuki ; Takahashi, Kenji. / Structural characterization of porcine enteropeptidase. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 31. pp. 19976-19982.
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