Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes

Kuniaki Mukai, Michiyo Imai, Hideo Shimada, Yukiko Okada, Tadashi Ogishima, Yuzuru Ishimura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Two rat genomic clones, one for cytochrome P-450aldo and the other for P45011β, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5′-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-45011β gene. S1 nuclease protection assay revealed a single transcription initiation site for the P450aldo gene, while multiple sites were found for the P-45011β gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-45011β genes is due to differences in the sequences of their 5′-flanking regions.

Original languageEnglish
Pages (from-to)1187-1193
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number3
DOIs
Publication statusPublished - Nov 14 1991
Externally publishedYes

Fingerprint

5' Flanking Region
Cytochromes
Rats
Genes
Nuclease Protection Assays
Gene encoding
Transcription Initiation Site
Cyclic AMP
Introns
Exons
Assays
Clone Cells
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes. / Mukai, Kuniaki; Imai, Michiyo; Shimada, Hideo; Okada, Yukiko; Ogishima, Tadashi; Ishimura, Yuzuru.

In: Biochemical and Biophysical Research Communications, Vol. 180, No. 3, 14.11.1991, p. 1187-1193.

Research output: Contribution to journalArticle

Mukai, Kuniaki ; Imai, Michiyo ; Shimada, Hideo ; Okada, Yukiko ; Ogishima, Tadashi ; Ishimura, Yuzuru. / Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 180, No. 3. pp. 1187-1193.
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