Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus

Daisuke Fujinami, Masaki Matsumoto, Takuya Noguchi, Kenji Sonomoto, Daisuke Kohda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An oligosaccharide chain attached to the asparagine residue in a structurally defined peptide was produced by an in vitro oligosaccharide- transfer reaction, using membrane fractions that contained the oligosaccharyltransferase from the hyperthermophilic archaeon, Pyrococcus furiosus. The chemical structure of the N-glycan was elucidated by sugar analysis, NMR spectroscopy, and MS spectrometry, which revealed the structure. The shorter glycan structures lacking one or two xylose residues were also transferred by the P. furiosus oligosaccharyltransferase. The archaeal N-glycans are known to exhibit a high degree of structural variation. The structure of the P. furiosus N-glycan is novel and unique. The present data will be useful for structural and functional studies of the P. furiosus oligosaccharyltransferase.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalCarbohydrate Research
Volume387
Issue number1
DOIs
Publication statusPublished - Mar 31 2014

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Pyrococcus furiosus
Asparagine
Archaea
Oligosaccharides
Polysaccharides
Xylose
Sugars
Spectrometry
Nuclear magnetic resonance spectroscopy
Spectrum Analysis
Magnetic Resonance Spectroscopy
Membranes
Peptides
dolichyl-diphosphooligosaccharide - protein glycotransferase

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus. / Fujinami, Daisuke; Matsumoto, Masaki; Noguchi, Takuya; Sonomoto, Kenji; Kohda, Daisuke.

In: Carbohydrate Research, Vol. 387, No. 1, 31.03.2014, p. 30-36.

Research output: Contribution to journalArticle

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