Structural gene of cytochrome b-562 from the cytochrome b-c1 complex of Rhodobacter sphaeroides

Koh Iba, Ken-Ichirou Morohashi, Toshiyuki Miyata, Ken ichiro Takamiya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The structural gene coding for cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodobacter (Rhodopseudomonas) sphaeroides has been cloned. Its nucleotide sequence has been determined and the amino acid sequence was deduced therefrom. It consists of 157 amino acids Mr 17,237) and contains four hydro-phobic segments. The first 30 residues in the predicted amino acid sequence are the same as those determined for the NH2,-terminal portion of purified cytochrome b-562. The amino acid composition is in accord with that determined for the pure protein. From the hydropathy profile and molar ratio of protoheme to cytochrome b-562, it is suggested that the structural and functional unit of the cytochrome is a two-heme cross-linked homodimer.

Original languageEnglish
Pages (from-to)1511-1518
Number of pages8
JournalJournal of Biochemistry
Volume102
Issue number6
DOIs
Publication statusPublished - Jan 1 1987

Fingerprint

Cytochromes c1
Rhodobacter sphaeroides
Cytochromes b
Genes
Amino Acids
Heme
Amino Acid Sequence
Cytochromes
Nucleotides
Chemical analysis
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Structural gene of cytochrome b-562 from the cytochrome b-c1 complex of Rhodobacter sphaeroides. / Iba, Koh; Morohashi, Ken-Ichirou; Miyata, Toshiyuki; Takamiya, Ken ichiro.

In: Journal of Biochemistry, Vol. 102, No. 6, 01.01.1987, p. 1511-1518.

Research output: Contribution to journalArticle

@article{4dd7dc9ba21446af86024752899a3948,
title = "Structural gene of cytochrome b-562 from the cytochrome b-c1 complex of Rhodobacter sphaeroides",
abstract = "The structural gene coding for cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodobacter (Rhodopseudomonas) sphaeroides has been cloned. Its nucleotide sequence has been determined and the amino acid sequence was deduced therefrom. It consists of 157 amino acids Mr 17,237) and contains four hydro-phobic segments. The first 30 residues in the predicted amino acid sequence are the same as those determined for the NH2,-terminal portion of purified cytochrome b-562. The amino acid composition is in accord with that determined for the pure protein. From the hydropathy profile and molar ratio of protoheme to cytochrome b-562, it is suggested that the structural and functional unit of the cytochrome is a two-heme cross-linked homodimer.",
author = "Koh Iba and Ken-Ichirou Morohashi and Toshiyuki Miyata and Takamiya, {Ken ichiro}",
year = "1987",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a122198",
language = "English",
volume = "102",
pages = "1511--1518",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Structural gene of cytochrome b-562 from the cytochrome b-c1 complex of Rhodobacter sphaeroides

AU - Iba, Koh

AU - Morohashi, Ken-Ichirou

AU - Miyata, Toshiyuki

AU - Takamiya, Ken ichiro

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The structural gene coding for cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodobacter (Rhodopseudomonas) sphaeroides has been cloned. Its nucleotide sequence has been determined and the amino acid sequence was deduced therefrom. It consists of 157 amino acids Mr 17,237) and contains four hydro-phobic segments. The first 30 residues in the predicted amino acid sequence are the same as those determined for the NH2,-terminal portion of purified cytochrome b-562. The amino acid composition is in accord with that determined for the pure protein. From the hydropathy profile and molar ratio of protoheme to cytochrome b-562, it is suggested that the structural and functional unit of the cytochrome is a two-heme cross-linked homodimer.

AB - The structural gene coding for cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodobacter (Rhodopseudomonas) sphaeroides has been cloned. Its nucleotide sequence has been determined and the amino acid sequence was deduced therefrom. It consists of 157 amino acids Mr 17,237) and contains four hydro-phobic segments. The first 30 residues in the predicted amino acid sequence are the same as those determined for the NH2,-terminal portion of purified cytochrome b-562. The amino acid composition is in accord with that determined for the pure protein. From the hydropathy profile and molar ratio of protoheme to cytochrome b-562, it is suggested that the structural and functional unit of the cytochrome is a two-heme cross-linked homodimer.

UR - http://www.scopus.com/inward/record.url?scp=0023481489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023481489&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a122198

DO - 10.1093/oxfordjournals.jbchem.a122198

M3 - Article

VL - 102

SP - 1511

EP - 1518

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 6

ER -