Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus

Immunocytochemical evidence from specific monoclonal antibodies

Takeshi Honda, Ayami Matsushima, Kazunori Sumida, Yoshiro Chuman, Kazuyasu Sakaguchi, Hitoshi Onoue, Ian A. Meinertzhagen, Yasuyuki Shimohigashi, Miki Shimohigashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pigment-dispersing factor (PDF) is an 18-mer peptide that acts as a principal neurotransmitter of the insect circadian clock. Our previous study, utilizing anti-Uca β-PDH polyclonal antibody (pAb) to immunolabel the optic lobe of the cricket Gryllus bimaculatus, suggested the existence of an alternative PDF-like peptide in the outer cells of the first neuropile, or lamina (La), which were much less immunoreactive than the inner cells of the second neuropile, the medulla (Me). To obtain structural information about such a PDF-like peptide, we prepared 10 anti-Gryllus PDF monoclonal (mAb) and pAb antibodies and analyzed their detailed epitope specificities. The PDFMe and PDFLa inner cells and their axonal projections were clearly immunoreactive to all these antibodies, revealing the widespread immunocytochemical organization of the PDF system in the optic lobe, as seen previously with anti-Uca β-PDH pAb and anti-Gryllus PDF mAb, the epitope structures of which were also clarified in this study. The lamina outer cells, which we found lacked a target pdf mRNA, displayed specific immunoreactivities, indicating that the cells contain a distinct PDF-like peptide possessing both N- and C-terminal structures. These cells were not immunolabeled by some other monoclonal antibodies, however, implying that the PDFLa outer cells have a PDF isoform peptide devoid of Asn at positions 6 and 16. This isoform was also identified in a varicose arborization in the lamina. These results suggest not only the structure of the peptide, but also the possibility of additional functions of this novel PDF isoform.

Original languageEnglish
Pages (from-to)404-421
Number of pages18
JournalJournal of Comparative Neurology
Volume499
Issue number3
DOIs
Publication statusPublished - Nov 20 2006

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Gryllidae
Neuropeptides
Protein Isoforms
Monoclonal Antibodies
Peptides
Brachyura
Neuropil
Antibodies
Epitopes
Circadian Clocks
Neurotransmitter Agents
Insects
Anti-Idiotypic Antibodies
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus : Immunocytochemical evidence from specific monoclonal antibodies. / Honda, Takeshi; Matsushima, Ayami; Sumida, Kazunori; Chuman, Yoshiro; Sakaguchi, Kazuyasu; Onoue, Hitoshi; Meinertzhagen, Ian A.; Shimohigashi, Yasuyuki; Shimohigashi, Miki.

In: Journal of Comparative Neurology, Vol. 499, No. 3, 20.11.2006, p. 404-421.

Research output: Contribution to journalArticle

Honda, Takeshi ; Matsushima, Ayami ; Sumida, Kazunori ; Chuman, Yoshiro ; Sakaguchi, Kazuyasu ; Onoue, Hitoshi ; Meinertzhagen, Ian A. ; Shimohigashi, Yasuyuki ; Shimohigashi, Miki. / Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus : Immunocytochemical evidence from specific monoclonal antibodies. In: Journal of Comparative Neurology. 2006 ; Vol. 499, No. 3. pp. 404-421.
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