Structural organization of the bovine adrenodoxin gene

Yasuhiro Sagara, Hiromi Sawae, Akihumi Kimura, Yasuko Sagara-nakano, Ken-Ichirou Morohashi, Ken Ichi Miyoshi, Tadao Horiuchi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The gene structure of bovine adrenodoxin (Ad), a component of the steroid hydroxylating system in adrenal cortex mitochondria, was determined. This gene seems to be a single copy, but contains a pseudo exon in addition to the genuine gene region. The gene region for the active Ad is divided into 4 exons by 3 introns, and spans at least 27 kb. All the splice donor and acceptor sites follow the GT/AG rule. The transcription initiation site was determined by primer extension analysis, and putative TATA and GC boxes are present in the 5'-flanking region. A 12 base sequence, CCAGGGCCAGGG, is present 95 bases upstream from the transcription initiation site. A similar sequence was also found in the 5'-flanking regions of all genes for the steroid hydroxylating cytochromes P-450: P-450(SCC), P-450(11 β), P-450(17α), and P-450(C21), in the adrenal cortex. The consensus sequence for these five sequences is C GCAGGGCCA TGGG A. The pseudo exon, exon 1', was present about 0.5 kb upstream from exon 1. It encodes the prepeptide region of the minor type Ad mRNA, but has a stop codon (TAA) in the frame. A TATA-box-like sequence and a CAAT-box-like sequence are also present in the 5'-flanking region of exon 1'. The GC content of exon 1 was over 80%, whereas for each of the other four exons, including exon 1', it was about 40%. Our observations suggest that exon 1, which encodes the prepeptide region of the major type Ad mRNA, has intruded into the region between exon 1' and exon 2.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalJournal of Biochemistry
Volume107
Issue number1
DOIs
Publication statusPublished - Jan 1 1990

Fingerprint

Adrenodoxin
Exons
Genes
5' Flanking Region
TATA Box
RNA Splice Sites
Transcription Initiation Site
Adrenal Cortex
Steroids
Messenger RNA
Mitochondria
Terminator Codon
Consensus Sequence
Base Composition
Introns
Cytochrome P-450 Enzyme System

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Sagara, Y., Sawae, H., Kimura, A., Sagara-nakano, Y., Morohashi, K-I., Miyoshi, K. I., & Horiuchi, T. (1990). Structural organization of the bovine adrenodoxin gene. Journal of Biochemistry, 107(1), 77-83. https://doi.org/10.1093/oxfordjournals.jbchem.a123015

Structural organization of the bovine adrenodoxin gene. / Sagara, Yasuhiro; Sawae, Hiromi; Kimura, Akihumi; Sagara-nakano, Yasuko; Morohashi, Ken-Ichirou; Miyoshi, Ken Ichi; Horiuchi, Tadao.

In: Journal of Biochemistry, Vol. 107, No. 1, 01.01.1990, p. 77-83.

Research output: Contribution to journalArticle

Sagara, Y, Sawae, H, Kimura, A, Sagara-nakano, Y, Morohashi, K-I, Miyoshi, KI & Horiuchi, T 1990, 'Structural organization of the bovine adrenodoxin gene', Journal of Biochemistry, vol. 107, no. 1, pp. 77-83. https://doi.org/10.1093/oxfordjournals.jbchem.a123015
Sagara, Yasuhiro ; Sawae, Hiromi ; Kimura, Akihumi ; Sagara-nakano, Yasuko ; Morohashi, Ken-Ichirou ; Miyoshi, Ken Ichi ; Horiuchi, Tadao. / Structural organization of the bovine adrenodoxin gene. In: Journal of Biochemistry. 1990 ; Vol. 107, No. 1. pp. 77-83.
@article{fe0060a5ea924549984c162bc51ab3f6,
title = "Structural organization of the bovine adrenodoxin gene",
abstract = "The gene structure of bovine adrenodoxin (Ad), a component of the steroid hydroxylating system in adrenal cortex mitochondria, was determined. This gene seems to be a single copy, but contains a pseudo exon in addition to the genuine gene region. The gene region for the active Ad is divided into 4 exons by 3 introns, and spans at least 27 kb. All the splice donor and acceptor sites follow the GT/AG rule. The transcription initiation site was determined by primer extension analysis, and putative TATA and GC boxes are present in the 5'-flanking region. A 12 base sequence, CCAGGGCCAGGG, is present 95 bases upstream from the transcription initiation site. A similar sequence was also found in the 5'-flanking regions of all genes for the steroid hydroxylating cytochromes P-450: P-450(SCC), P-450(11 β), P-450(17α), and P-450(C21), in the adrenal cortex. The consensus sequence for these five sequences is C GCAGGGCCA TGGG A. The pseudo exon, exon 1', was present about 0.5 kb upstream from exon 1. It encodes the prepeptide region of the minor type Ad mRNA, but has a stop codon (TAA) in the frame. A TATA-box-like sequence and a CAAT-box-like sequence are also present in the 5'-flanking region of exon 1'. The GC content of exon 1 was over 80{\%}, whereas for each of the other four exons, including exon 1', it was about 40{\%}. Our observations suggest that exon 1, which encodes the prepeptide region of the major type Ad mRNA, has intruded into the region between exon 1' and exon 2.",
author = "Yasuhiro Sagara and Hiromi Sawae and Akihumi Kimura and Yasuko Sagara-nakano and Ken-Ichirou Morohashi and Miyoshi, {Ken Ichi} and Tadao Horiuchi",
year = "1990",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a123015",
language = "English",
volume = "107",
pages = "77--83",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Structural organization of the bovine adrenodoxin gene

AU - Sagara, Yasuhiro

AU - Sawae, Hiromi

AU - Kimura, Akihumi

AU - Sagara-nakano, Yasuko

AU - Morohashi, Ken-Ichirou

AU - Miyoshi, Ken Ichi

AU - Horiuchi, Tadao

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The gene structure of bovine adrenodoxin (Ad), a component of the steroid hydroxylating system in adrenal cortex mitochondria, was determined. This gene seems to be a single copy, but contains a pseudo exon in addition to the genuine gene region. The gene region for the active Ad is divided into 4 exons by 3 introns, and spans at least 27 kb. All the splice donor and acceptor sites follow the GT/AG rule. The transcription initiation site was determined by primer extension analysis, and putative TATA and GC boxes are present in the 5'-flanking region. A 12 base sequence, CCAGGGCCAGGG, is present 95 bases upstream from the transcription initiation site. A similar sequence was also found in the 5'-flanking regions of all genes for the steroid hydroxylating cytochromes P-450: P-450(SCC), P-450(11 β), P-450(17α), and P-450(C21), in the adrenal cortex. The consensus sequence for these five sequences is C GCAGGGCCA TGGG A. The pseudo exon, exon 1', was present about 0.5 kb upstream from exon 1. It encodes the prepeptide region of the minor type Ad mRNA, but has a stop codon (TAA) in the frame. A TATA-box-like sequence and a CAAT-box-like sequence are also present in the 5'-flanking region of exon 1'. The GC content of exon 1 was over 80%, whereas for each of the other four exons, including exon 1', it was about 40%. Our observations suggest that exon 1, which encodes the prepeptide region of the major type Ad mRNA, has intruded into the region between exon 1' and exon 2.

AB - The gene structure of bovine adrenodoxin (Ad), a component of the steroid hydroxylating system in adrenal cortex mitochondria, was determined. This gene seems to be a single copy, but contains a pseudo exon in addition to the genuine gene region. The gene region for the active Ad is divided into 4 exons by 3 introns, and spans at least 27 kb. All the splice donor and acceptor sites follow the GT/AG rule. The transcription initiation site was determined by primer extension analysis, and putative TATA and GC boxes are present in the 5'-flanking region. A 12 base sequence, CCAGGGCCAGGG, is present 95 bases upstream from the transcription initiation site. A similar sequence was also found in the 5'-flanking regions of all genes for the steroid hydroxylating cytochromes P-450: P-450(SCC), P-450(11 β), P-450(17α), and P-450(C21), in the adrenal cortex. The consensus sequence for these five sequences is C GCAGGGCCA TGGG A. The pseudo exon, exon 1', was present about 0.5 kb upstream from exon 1. It encodes the prepeptide region of the minor type Ad mRNA, but has a stop codon (TAA) in the frame. A TATA-box-like sequence and a CAAT-box-like sequence are also present in the 5'-flanking region of exon 1'. The GC content of exon 1 was over 80%, whereas for each of the other four exons, including exon 1', it was about 40%. Our observations suggest that exon 1, which encodes the prepeptide region of the major type Ad mRNA, has intruded into the region between exon 1' and exon 2.

UR - http://www.scopus.com/inward/record.url?scp=0025117560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025117560&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a123015

DO - 10.1093/oxfordjournals.jbchem.a123015

M3 - Article

VL - 107

SP - 77

EP - 83

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 1

ER -