Structural organization of the mouse mitochondrial malate dehydrogenase gene

Hideo Takeshima, Tadashi Joh, Teruhisa Tsuzuki, Kazunori Shimada, Yasuhiko Matsukado

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Structural organization of the mouse mitochondrial malate dehydrogenase (EC 1.1.1.37) gene was determined by analyzing a genomic DNA fragment isolated from a cosmid library. The gene is 12,000 base-pairs long and contains nine exons interrupted by eight introns of various sizes. The 5′ and 3′-flanking regions, and the exact sizes and boundaries of the exon blocks including the transcription-initiation sites were determined. In the 5′-flanking region, there is neither a TATA box nor a CAAT box. Instead of these sequences, there are six copies of the GGGCGG or CCGCCC sequence, which is a potential binding site for the transcription factor, Spl. The 5′-flanking region up to about 600 nucleotides is G + C-rich (65%) and contains sequences compatible with the formation of a number of potentially stable stem-loop structures. S1 nuclease mapping and primer extension analysis demonstrated that transcription of the mitochondrial malate dehydrogenase gene initiates at multiple sites. Comparison of the nucleotide sequence of the promoter region of the mitochondrial malate dehydrogenase gene with that of the mitochondrial aspartate aminotransferase gene, revealed that there are several highly conserved regions between these two mitochondrial enzyme genes participating in the malate-aspartate shuttle.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of Molecular Biology
Volume200
Issue number1
DOIs
Publication statusPublished - Mar 5 1988

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Malate Dehydrogenase
5' Flanking Region
Genes
Exons
Mitochondrial Aspartate Aminotransferase
3' Flanking Region
Cosmids
TATA Box
Mitochondrial Genes
Transcription Initiation Site
Genetic Promoter Regions
Aspartic Acid
Base Pairing
Introns
Transcription Factors
Nucleotides
Binding Sites
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Structural organization of the mouse mitochondrial malate dehydrogenase gene. / Takeshima, Hideo; Joh, Tadashi; Tsuzuki, Teruhisa; Shimada, Kazunori; Matsukado, Yasuhiko.

In: Journal of Molecular Biology, Vol. 200, No. 1, 05.03.1988, p. 1-11.

Research output: Contribution to journalArticle

Takeshima, H, Joh, T, Tsuzuki, T, Shimada, K & Matsukado, Y 1988, 'Structural organization of the mouse mitochondrial malate dehydrogenase gene', Journal of Molecular Biology, vol. 200, no. 1, pp. 1-11. https://doi.org/10.1016/0022-2836(88)90328-2
Takeshima, Hideo ; Joh, Tadashi ; Tsuzuki, Teruhisa ; Shimada, Kazunori ; Matsukado, Yasuhiko. / Structural organization of the mouse mitochondrial malate dehydrogenase gene. In: Journal of Molecular Biology. 1988 ; Vol. 200, No. 1. pp. 1-11.
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